...improving access to useful research materials and information. We will pursue opportunities to build on our...

.... This provides new (and old) employees with information on how Addgene operates as a team and shows how...

...sensitive than green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows...

...showed that different fluorescently labeled dCas9 isoforms are efficiently directed to the proper target ...

...highlight different skills and to get the important information in the right place on the page. 5) Apply for ...

...B3 recombinases from the Rubin lab For more information about limitations and caveats of Cre usage: Vagaries...

...include in their annual NIH progress reports information on the Individual Development Plans (IDPs) for...

... my previous post if you're looking for more information about the benefits of pursuing extracurricular...

...bacterial systems. Plasmids with wild-type Cas13a (formerly C2c2) can mediate targeted RNA knockdown, which...

... my peers. In fact, there was so much useful information that I decided to write a few blog posts. This...

...of University of Michigan says, “Addgene has transformed the way in which we share plasmids and other ...

...candidate emerges, downstream optimization is performed to take the drug to the pre-clinical and human...

...yourself taking? If so, consider setting up an informational interview with them to learn how their master's...

...downstream applications. Tag cleavage must be performed after primary purification and requires a second...

...elevated temperatures. In both cases, GeoCas9 outperformed SpCas9 at high temperatures. See Figure 1 for...

...Genetically Encoded H2O2 Probe with Improved Performance for Ratiometric and Fluorescence Lifetime Imaging...

... Sometimes this means you have to fire a good performer if they are enough of a jerk to make the rest ...

...create a new strain with a disrupted gene by transforming wild-type yeast with an integrating vector expressing...

...pro in a field, but to be trained to think and perform like a scientist. Researchers should  pick a topic...

...protocols and performing rigorous quality control (QC). The specific QC experiments performed varies for ...Link opens in a new window) . Transfections are performed using the transfer plasmid, a plasmid encoding...and stored at -80 °C. Titer Titering is either performed by Addgene or by the University of Pennsylvania...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Pennsylvania Vector Core is (as of April 2016) also performed by droplet digital PCR (ddPCR). Quality Control.... To learn which specific QC experiments were performed on your lot, please contact us . Full sequencing...the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final AAV preparation...