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  1. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...attractive features is its stability and easily obtainable growing conditions. As a facultative aerobe, ...regenerate complex structures such as limbs, the spinal cord, and internal organs including the heart, ...
  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous protein tagging...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent...
  3. Finding Your Science Policy Path

    Type
    Blog Post
    ...with early career science policy groups, attend webinars, and learn more about the field.  Read the CCST...accelerator for some hands-on guidance.   Check out my webinar via the NC Science Policy student group SciPolPack...
  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...variable) Sequence-level data Low-throughput, inappropriate for heterogenous populations, low sensitivity...given the stricter sequence requirement at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al....
  5. Typing CRISPR Systems

    Type
    Blog Post
    ...The current classification system relies on a combination of sequence similarity, phylogenetic analysis..., & Ke, A. (2024). Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing...
  6. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...use, including all information provided in the original reference library, as well as GC%, reverse compliment... that you have specified.   Run your screen! Final note I am very grateful to the researchers letting...
  7. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...and fluorescent light sources. Visible light illuminates each cell as it passes through and scatters in...staining is quicker than indirect staining and eliminates potential non-specific staining that may arise...
  8. New Coomassie Protocol Video

    Type
    Blog Post
    ...latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."  Hot tip! This protocol can easily...
  9. Antibodies 101 Series on TikTok

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    Blog Post
    ...antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations...
  10. Addgene's 20th Anniversary Party

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    Blog Post
    ...even made an appearance during co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia...
  11. The 12 Days of CRISPR

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    Blog Post
    ...prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR...
  12. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...community, we've partnered with distributors in China, Korea, Japan, India, and Brazil.  We've also created...
Showing: 761 - 780 of 929 results