... Human M3 muscarinic Arrestin-2/-3 CNO* Increase Arrestin translocation Arrestin signalling Nakajima &...used. There is also a DREADD that couples to β-arrestin to activate noncanonical GPCR signalling independent...used to target AAV vectors to specific cells of interest. In addition, different AAV serotypes have varying...Design and functional characterization of a novel, arrestin-biased designer G protein-coupled receptor. Mol... Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function. Cell Stem Cell 28:112-126.e6. ...

...transgene of interest is cloned into the shuttle vector, verified, and linearized with the restriction enzyme...plasmids are then verified for size and proper restriction digest patterns to determine that the transgene...enables co-expression of GFP with the transgene of interest. With these plasmids, one can track the infection...

... the majority of the genome, while others are restricted to certain gene sets. In cDNA libraries, each... DNA can be delivered directly to the cells of interest. With others, notably pooled lentiviral plasmid...subsequently used to deliver the plasmids to the cells of interest. In either case, next-generation sequencing of...

...conditions: The sequence is unique compared to the rest of the genome The target is present immediately ...Publications CRISPR Overview Bacteria have an interesting adaptive immune response that detects and eliminates... Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20...activity with endogenous cell-type specific miRNAs to restrict CRISPR editing only to the target tissue. Browse... molecules associated with a genomic region of interest in vivo. Figure 12: Overview of purifying genomic...

...Learn about plasmids, sequencing, vectors, and restriction enzymes to assist with your molecular biology...Plasmid Cloning Creating Bacterial Glycerol Stocks Restriction Digests Guides Browse our in-depth instructional...

...to capture, quantify, and identify proteins of interest. Read More Chemogenetics Chemogenetics is a technique... the majority of the genome, while others are restricted to certain gene sets. Read More Viral Vectors...

...controls when and where in the organism your gene of interest is expressed. Summary Promoters are about 100-...

...utilized to control the localization of a protein of interest. A genetically-encoded nuclear Cry2 fusion can...