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  4. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...and see if we should start celebrating papers and grants too with pizza or pub.” The Cepko Lab celebrates... all work and no play Although hard work and perseverance are required to achieve success in the lab, ...

  7. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...with a detailed training schedule that helped me transition easily. Training and responsibilities Through... the “9-5” was not as scary as it seemed. The transition into a traditional work environment was aided...

  8. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...released the MORF collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500...conduct robust investigations into the human transcriptome. The MORF library is only one of 22 libraries...

  9. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...many applications, the most well-known being transcriptional activation/repression. dCas9 has also previously...for example, the visualization of chromosomal translocations or chromosomal shattering (chromothripsis)....

  13. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...from the DNA. These apurinic sites if in double stranded DNA are repaired by base excision repair, but ...but can lead to mutations in single stranded DNA undergoing replication.   DNase on the other hand can digest...

  14. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...beyond your immediate field, fostering a diverse range of perspectives and sparking innovative thinking...Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple of postdocs...

  16. Hot Plasmids - October 2022

    Type
    Blog Post
    ...voltage indicator: JEDI-2P  by: Brian O'Neill  The François St-Pierre lab and collaborators have successfully...photoactivation of HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1...

  20. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...joyful that getting to the final step and the transformed bacteria refuse to grow. Eventual success was...
Showing: 741 - 760 of 986 results