We narrowed to 913 results for: plasmid

Learn about the structure and life cycle of retroviruses to help understand how to use viral vectors tools in the lab.

Two new CRISPR tools from the Abudayyeh-Gootenberg lab are now available at Addgene: Cas7/11 and PASTE.

An introduction to the use of antibodies in immunoprecipitation, including antibody immobilization, controls, and a basic overview of techniques.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Marc Zimmer's book, The State of Science, examines broad issues in science including racism and sexism, peer-review, funding, CRISPR, and the politicization of science.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

Find tips and tricks for using digital droplet PCR to quantify your AAV titers.

Crossing the blood brain barrier is difficult for many AAVs. But, PHP.B has impressive CNS transduction in some mice. Learn more about how PHP.B and about its strain specific performance.

Electronic lab notebooks (ELNs) are useful tools that help researchers keep track of data. However, not all ELNs support the same functionality when it comes to reproducibility.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Learn how and why Adeno-associated virus (AAV) has enabled breakthroughs in retinal gene therapy.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

Learn all about mesothelioma and upcoming immunotherapy treatments for this devastating form of cancer.

Luke Lavis from Janelia Farms describes how small molecule fluorophores can still be useful in the age of fluorescent proteins.

Learn how to design a CRISPR screening experiment targeting enhancers.

Learn about the founding the science communication nonprofit (CINSO) and get tips on thinking about your science career.

Learn how the team building atmosphere at Addgene keeps its employees excited about helping researchers share their science.

Tips on designing primers for PCR

If flow plots seem confusing, our guide to understanding them is here to help!

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.