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  1. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...team discussions can often lead to otherwise unobtainable solutions. To some extent there is the need ...role Talking to customers everyday, it can be fascinating and exciting to listen to the many individual...

  2. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...codons, the ribosome may therefore stall at inappropriate locations or fail to make it through the entire...and is folding properly. References 1. Angov, Evelina, et al. "Heterologous protein expression is enhanced...

  3. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...before you know your system is working as expected. Finally, for each gene you want to activate, you should...study in cis regulation and also suffer from an inability to easily deliver the appropriate isoform(s) of...

  5. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...BioRender.com   The first couple of steps are preliminary steps that are not exclusive to IF, but they ...Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

  6. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...attractive features is its stability and easily obtainable growing conditions. As a facultative aerobe, ...regenerate complex structures such as limbs, the spinal cord, and internal organs including the heart, ...

  7. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... The diversity panel - Equity and inclusion The final and possibly the most important panel of the symposium...quantifying, and preventing this type of failure. Final thoughts The FOR Symposium Boston 2015 shined a ...

  8. Five Popular Model Organisms

    Type
    Blog Post
    ...actually two distinct populations of β-cells that originate in different regions of the pancreas and produce...1935914. PubMed Central PMCID: PMC453137. Menezes, Regina, et al. "From the baker to the bedside: yeast models...

  9. March for Science

    Type
    Blog Post
    ...level you can click on your state in this map. Finally, the march is meant to inspire scientists to be...Environmental Science and Technology: Crossing The Imaginary Line The New York Times: A Scientists’ March on...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous protein tagging...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent...

  11. Finding Your Science Policy Path

    Type
    Blog Post
    ...with early career science policy groups, attend webinars, and learn more about the field.  Read the CCST...accelerator for some hands-on guidance.   Check out my webinar via the NC Science Policy student group SciPolPack...

  12. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...variable) Sequence-level data Low-throughput, inappropriate for heterogenous populations, low sensitivity...given the stricter sequence requirement at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al....

  13. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...and fluorescent light sources. Visible light illuminates each cell as it passes through and scatters in...staining is quicker than indirect staining and eliminates potential non-specific staining that may arise...

  15. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...use, including all information provided in the original reference library, as well as GC%, reverse compliment... that you have specified.   Run your screen! Final note I am very grateful to the researchers letting...

  16. New Coomassie Protocol Video

    Type
    Blog Post
    ...latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."  Hot tip! This protocol can easily...

  17. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations...

  18. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...even made an appearance during co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia...

  19. The 12 Days of CRISPR

    Type
    Blog Post
    ...prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR...
Showing: 741 - 760 of 911 results