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  1. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post
    ...is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential...particle: pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences...

  2. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...actively use tools designed to help reduce or eliminate bias, just like the ones made available through.../s41586-022-04601-8 Lewis, Anna CF, Santiago J. Molina, Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo,...

  5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post
    ..., such as knockout generation or homologous recombination.  However, experiments that require long-term... laboratory reagents, equipment, and expertise. Final thoughts The Cas9-gRNA RNP method provides scientists...

  7. A Career in Grants: How To Become a Grant Professional

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    Blog Post
    ...grant professional’s job duties can range from coordinating the grant application and submitting it, to ... with research grants? Are you interested in sustainable community development? Volunteer as a grant writer...

  8. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...This article was originally published on the Society for Laboratory Automation and Screening (SLAS) Electronic... family member, want to train for a triathlon, finally buy that charming fixer-upper house you have always...

  9. Career Insights: Technical Support Specialist

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    Blog Post
    ...team discussions can often lead to otherwise unobtainable solutions. To some extent there is the need ...role Talking to customers everyday, it can be fascinating and exciting to listen to the many individual...

  10. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...GCaMP6 and jGCaMP7 do not. Most likely, it’s a combination of many factors unique to this set of tools and...quickly. Beyond the service This brings us to the final piece of the viral vectors service puzzle: the usefulness...

  11. Tips for Writing a Good Cover Letter

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    Blog Post
    ...with qPCR would make it easy to master ddPCR.  Finally, it’s a good idea to state why you’re applying ... you're ready to go! Good luck! This post was originally written by Maria Soriano-Carot in September 2016...

  12. Save Time with Transient Plant Leaf Transformations

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    Blog Post
    ...vinblastine and vincristine, are regulated in the medicinal plant, the Catharanthus roseus (Figure 1). The...antibodies in plants using syringe agroinfiltration." Recombinant proteins from plants. Humana Press, 2009. 41-...

  14. Plasmids 101: Codon usage bias

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    Blog Post
    ...codons, the ribosome may therefore stall at inappropriate locations or fail to make it through the entire...and is folding properly. References 1. Angov, Evelina, et al. "Heterologous protein expression is enhanced...

  15. CRISPR Activation: A Practical Guide

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    Blog Post
    ...before you know your system is working as expected. Finally, for each gene you want to activate, you should...study in cis regulation and also suffer from an inability to easily deliver the appropriate isoform(s) of...

  16. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...factors need to be controlled for in order to eliminate any alternative interpretation of the results.... shown in Figure 2, this was likely due to the original transfection conditions. Now that we have successfully...

  17. March for Science

    Type
    Blog Post
    ...level you can click on your state in this map. Finally, the march is meant to inspire scientists to be...Environmental Science and Technology: Crossing The Imaginary Line The New York Times: A Scientists’ March on...

  18. Five Popular Model Organisms

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    Blog Post
    ...actually two distinct populations of β-cells that originate in different regions of the pancreas and produce...1935914. PubMed Central PMCID: PMC453137. Menezes, Regina, et al. "From the baker to the bedside: yeast models...

  19. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...attractive features is its stability and easily obtainable growing conditions. As a facultative aerobe, ...regenerate complex structures such as limbs, the spinal cord, and internal organs including the heart, ...

  20. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ...BioRender.com   The first couple of steps are preliminary steps that are not exclusive to IF, but they ...Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...
Showing: 741 - 760 of 929 results