Addgene

We narrowed to 802 results for: Eng

Showing: 741 - 760 of 802 results

Results per page:

Tips to Make the Most of a Scientific Conference

Type

Blog Post

.... This made sure I didn’t zone out and kept me engaged throughout the day. After the conference If there...
Of Myc and Men

Type

Blog Post

...making the antibody more amenable to protein engineering. As a result, you can now find Myc1-9E10 in a...
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Type

Blog Post

...access to routinely, and it can be especially challenging when trying to develop antibodies against unstable...
Polymerase Chain Reaction Overview and Applications

Type

Blog Post

...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...
Hot Plasmids and Viral Preps - May 2021

Type

Blog Post

...(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng's lab now available as a ready-to-use viral prep ...
Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...
Plasmids 101: Optimizing Plasmid Yields

Type

Blog Post

...of endA+ strains Strains like Stbl3 have been engineered to reduce plasmid recombination, but are also...
Experimenting with New Careers while in Grad School

Type

Blog Post

...realized I wanted a career in science outreach: engaging the public to promote an awareness and understanding...
Genome-wide Screening Using CRISPR

Type

Blog Post

...multiple replicates and several cell types can strengthen your results. Enrichment or depletion of multiple...
Light Sheet Fluorescence Microscopy

Type

Blog Post

...C.D. Hojer, J.G. Egen, F. Hellal, F. Bradke, M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983...
Tips for Using BLAST to Verify Plasmids

Type

Blog Post

...included in the search to maximize the alignment length. While this recommendation is not infallible, we...
Simplify Cloning with in vivo Assembly

Type

Blog Post

...temperature (Tm) of around 60 °C for all primers. The length of the homologous region should be around 20 bp...
Getting to Know Expansion Microscopy

Type

Blog Post

...traditional full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...
Better Dyeing Through Chemistry & Small Molecule Fluorophores

Type

Blog Post

...motif attached to a fluorophore (Figure 1b). Engineered ligases (e.g., lipoic acid ligase, biotin ligase...
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Type

Blog Post

...beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing techniques function...
Plasmids 101: Colony PCR

Type

Blog Post

...Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR Additional Resources...
A Career in Grants: How To Become a Grant Professional

Type

Blog Post

...Becoming a grant professional is rewarding and challenging! Jo Miller, GPC, CSMS is the founder and principal...
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Type

Blog Post

...that provides a community for biologists and bioengineers to share their educational materials, protocols...
Your Lentiviral Plasmid FAQs Answered

Type

Blog Post

...the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target cells and...

Showing: 741 - 760 of 802 results

Results per page:

Tips to Make the Most of a Scientific Conference

Of Myc and Men

No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Polymerase Chain Reaction Overview and Applications

Hot Plasmids and Viral Preps - May 2021

Cloning Mammalian Cells with the Agarose Method

Plasmids 101: Optimizing Plasmid Yields

Experimenting with New Careers while in Grad School

Genome-wide Screening Using CRISPR

Light Sheet Fluorescence Microscopy

Tips for Using BLAST to Verify Plasmids

Simplify Cloning with in vivo Assembly

Getting to Know Expansion Microscopy

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Better Dyeing Through Chemistry & Small Molecule Fluorophores

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Plasmids 101: Colony PCR

A Career in Grants: How To Become a Grant Professional

Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Your Lentiviral Plasmid FAQs Answered

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections