... your field of view is centered on a region of interest. If needed, you can also use the fine focus knob...

...entire signaling pathways, but what if you’re interested in studying signaling pathways? Here’s where ...

...quantification). But what if you’ve got several interesting protein targets and you want to know if they ...

...parts, both of which must be present for a gene of interest to be expressed. These permit exquisite control...

...making specific knock-out edits in a gene(s) of interest and has an average off-target frequency of 5%....

...description to watch any sections you’re particularly interested in, but I’d recommend watching the whole livestream...

... aspects) applicable to industry if this path interests you  (e.g. human disease or models of disease,...

...the non-profit IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as well as teaching...

...Transfections allow for transient expression of a gene of interest in cell culture. This protocol describes how to...

...Agarose Gel Electrophoresis You may also like... Restriction Digest of Plasmid DNA Purifying DNA from an Agarose...

...colonies of bacteria containing your plasmid of interest. Add 1.5 mL of the stock culture to a 1.75 mL ...

... available. Tailed t-tests If you are solely interested in whether or not two samples are statistically...

... 14–30: Analyze and expand monoclonal lines of interest Equipment Class II, Type A2 Biological Safety ...

...factors are directly injected into the organ of interest for in vivo transdifferentiation. Multiple cell...

...pRC (Rep-Cap), plasmid expressing your gene of interest Triton X-100 Benzonase/DNAse I (Millipore 71205...

...respectively. The use of far-red light is of particular interest since blue light has limited applications in vivo...

... In restriction cloning, scientists utilize specific restriction enzymes to cut dsDNA of interest into...used to create recombinant DNA. Restriction Enzyme Cloning Restriction enzyme (endonuclease) based molecular... find a protocol for restriction cloning and an in-depth breakdown of restriction digests on our website...website. Restriction cloning of your gene of interest (YGOI) into a recipient plasmid. (Image from Plasmid...Empty Backbone Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO Gibson Type IIS Ligation ...scientists can amplify and manipulate genes of interest and then insert them into plasmids for replication...reasons, remains one of the most popular today. Restriction enzymes, which are naturally produced by certain...

...protein of interest. Signal strength is determined by how many antibodies bind the protein of interest, while... antibody of interest to a second, independent antibody for the same protein of interest, with no overlapping...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called... of the animal’s immune system. The antigen of interest is introduced to the animal, which then mounts...primary antibody which is bound to your protein of interest. Secondary antibodies can be isotype-specific ...recognize an available epitope on your protein of interest. Polyclonal antibodies are typically collected...Western blots should be run with your protein of interest and at least one ubiquitously expressed protein...

...contains several restriction enzyme sites, enabling easy insertion of DNA by restriction enzymes digestion...used for cloning by restriction enzyme digestion. For more information about restriction enzymes check out...human genome. Digesting the human genome with restriction enzymes would yield about two million DNA fragments...plasmid. By the 1970s the combined discoveries of restriction enzymes, DNA ligase, and gel electrophoresis ...molecule, which can be used to study DNA fragments of interest, such as genes. Creation of recombinant DNA Molecular...antibiotic-resistance gene, and at least one unique restriction enzyme recognition site. These elements allow... not carrying the plasmid. Additionally, the restriction enzyme site(s) allow for the cloning of a fragment...

...nucleotide edits that introduce a novel restriction site. The region of interest is PCR amplified, digested with...region of interest can be PCR amplified using primers that (A) flank the region of interest (deletions...necessary to model your specific disease or process of interest. Do you want to: Generate complete and permanent...the promoter driving expression of your gene of interest. For genetic knockouts, gRNAs commonly target ... containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact cloning ... within a mixed cell population. The region of interest is PCR amplified, PCR products are denatured-renatured...agarose gel to identify DNA fragments. PCR and restriction digest (for HDR repaired DSBs): For small nucleotide...