Addgene

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Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Type

Blog Post

... protocols, including making LB agar plates, restriction digest of plasmid DNA, and bacterial transformation...
Plasmids 101: Stringent Regulation of Replication

Type

Blog Post

...levels low even if plasmid copy numbers are high, restraining further replication. Figure 3: Comparison...
Hot Plasmids Spring 2024

Type

Blog Post

...extraneous genetic material to reconstitute the gene of interest, reducing the immunogenicity typically associated...
Transferable Skills Guide: Creativity

Type

Blog Post

...exercise: Consider the issue as if there were no restraints on your problem-solving resources. What would...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Type

Blog Post

...they naturally find people with which they have interests and experiences in common. We often refer to this...
CRISPR Activation: A Practical Guide

Type

Blog Post

... you can achieve using a CRISPRa. Your gene of interest might already be hitting an upper bound of activation...
Tips for CRISPR Gene Editing in Mice

Type

Blog Post

... delete the entire gene? Perhaps you are more interested in the c-terminus and would like to target that...
Hot Plasmids - November 2023

Type

Blog Post

...Credit: Zhuhao Wu, Weill Cornell Medicine. Interested in this antibody, but wish it were from a different...
Neuronal labeling with Spaghetti Monster

Type

Blog Post

...antigenic peptide sequences, attached to a protein of interest (POI), that facilitate immunohistochemistry (IHC...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

... Massachusetts General Hospital with research interests in the fields of gene editing and stem cells. ...
Changing Labor Laws Bring Increased Postdoc Wages

Type

Blog Post

...Executive Director of Future of Research. He is interested in advocating for and with junior scientists,...
Healthcare Consulting: A Door to the Business of Life Sciences

Type

Blog Post

...works as a healthcare consultant in Boston. He is interested in the business of life sciences and it's impact...
The Challenges of Cell Culture

Type

Blog Post

... there would be no viable viruses to study. Interestingly, due to certain high profile problems surrounding...
Fluorescence Titering Assay

Type

Protocol

...with multiple integration events leading to underestimation of the true titer. Calculate the transduction...
An Integrin Antibody Toolkit from IPI

Type

Blog Post

...preferences of the αV subunit and its β partners. Of interest, we demonstrated that anti-integrin αVβ5.9 “essentially...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

..., the partial genomes complement each other to restore the full-expression cassette. AAV’s natural ability...
Transferable Skills Guide: Time Management

Type

Blog Post

...Gilmore directly to see if he knows anyone who’s interested in meeting Send out as many emails as possible...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Type

Blog Post

...reads that we studied by deep sequencing, but interestingly (and to our frustration) in many cases we only...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...viral protein production, so researchers are also interested in precisely excising the HIV-1 genome from infected...
How to Negotiate a Successful Lab Start Up Budget

Type

Blog Post

...This is actually why a department/institute is interested in you. Your science will define your success...

Showing: 701 - 720 of 748 results

Results per page:

Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Plasmids 101: Stringent Regulation of Replication

Hot Plasmids Spring 2024

Transferable Skills Guide: Creativity

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

CRISPR Activation: A Practical Guide

Tips for CRISPR Gene Editing in Mice

Hot Plasmids - November 2023

Neuronal labeling with Spaghetti Monster

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Changing Labor Laws Bring Increased Postdoc Wages

Healthcare Consulting: A Door to the Business of Life Sciences

The Challenges of Cell Culture

Fluorescence Titering Assay

An Integrin Antibody Toolkit from IPI

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Transferable Skills Guide: Time Management

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

How to Negotiate a Successful Lab Start Up Budget

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