...faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139... T7 RNAP expression alleviates inclusion body formation. Expression vector cannot have p15A origin of ...

...the probe for their needs. You’ll find basic information on NIR FPs, their properties and applications...mIFP6. Learn more about the characteristics, performance, and applications of near-infrared calcium indicators...

... use, but when you need to juggle filming and performing on camera, it’s a lot to handle. We decided the...talent could just focus on their interview or performance. By this point, towards the end of the summer...

.... I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of his/her... I would not recommend putting these into slide format. You want people to focus on you and your ideas...

...day. Note that intracellular labeling must be performed only after viability staining and surface labeling...surface labeling. Intracellular labeling can be performed on cytosolic and/or nuclear markers. Lastly, stains...

...found that many researchers feel that they lack formal training in a variety of transferable skills. At..., the task will likely be much easier. Having a formal plan for your day will make this easier - if you...

...hour or so, the sample is immediately ready to transform into competent cells. The master mix of enzymes...Gibson assembly master mix for 1 hour, and then transform into competent cells. The success rate of this...

...submerged in a tank filled with a transfer buffer formulated to conduct the electric current. The wet transfer...addition, the thickness of the band provides information about the relative abundance of the protein in...

...of one AAV serotype with the capsid of another. Formally, pseudotypes are described with two numbers separated...using the genomic structure of AAV2, so they are formally called rAAV2/1, rAAV2/2, and so on, although we...

...can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations in animal models or cells...piggyBac™ for gene editing allows you to efficiently perform seamless genetic manipulations, while avoiding ...

...scholar/student: visiting from another university to perform research   Why should I try research? Personally...reflect on what I wanted for my own future. Informational interviews with people who have taken career...

... studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used to rapidly...to screen additional ligases, conditions, and formulations and has led to the development of the new HiFi...

...and Agrobacterium-mediated transformation are available. For more information on this system, check out...

...certain circumstances) Current base editors can perform any base-to-base conversion in principle, but some...the process of double nicking increases indel formation slightly. Designing the sgRNA with a spacer that...

... minimal limitations. Background In its simplest form, PCR based cloning is about making a copy of a piece...recipient plasmid backbone. Transformation Proceed with the transformation according to the manufacturer...from your transformation will give you the first indication as to whether your transformation worked. Your...Digest of Plasmid DNA DNA Ligation Bacterial Transformation Summary PCR based cloning is incredibly versatile...see (Link opens in a new window) NEB for more information). Thus, we recommend that you add 3-6 bases upstream...should ensure that the bases do not result in the formation of a hairpin structure within your primer. In ...competent cells. For most standard cloning, you can transform 1-2μl of your ligation reaction into competent...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium...specific molecules or other factors. Most often, a conformational change in the sensing region of the construct...Citron/Citroff), mitochondrial or cytosolic High-Performance Intensiometric Direct- and Inverse-Response Genetically...

...our case 220 mL) to the same bottle and swirl to form a medium/agar colloid. Cover the opening of the ...lab. The addition of agar to LB results in the formation of a gel that bacteria can grow on, as they are...

...and left undisturbed for 13 days. (a, b) Colonies formed in individual wells of a 96-well plate after expansion...monoclonal cell lines. When this protocol was performed at Addgene on the A549 cells described in our ...expression and/or other phenotypes. For example, perform Western blotting to screen for lines with the highest...blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts from the initial stable...

...Antibodies 101 blog series.  Stay tuned for more information on our blog and social media! ...

... of vectors, capsids, and tools; and helpful information on everything from producing your own vectors...