Skip to main content
Addgene
Showing: 701 - 720 of 929 results

  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...dCas9 fused to the Activation Induced Cytidine Deaminase (AID) enzyme. AID, however, only changes cytidines...targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard mutation ...

  4. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...applications Vaccine development Attenuated and inactivated viruses pioneered vaccine development well over...

  5. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward across...for multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus Pros Cons ...

  6. Hot Plasmids - February 2022

    Type
    Blog Post
    ...identifying guide selection rules, and reducing recombination by finding acceptable variations in DR sequences... cassette provides increased simplicity for combinatorial genetic screens, and this optimized toolkit ...

  7. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...dissociation from the GPCR allows for binding of a new inactive heterotrimeric G protein complex and subsequently...pathways from ligand to receptor to G-protein, and finally, to a response? Let’s take a look at a few examples...

  9. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...Ku then acts as a scaffold for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-...reduces breaks and mutations at off-target sites. Christina Mork contributed to the update of this post. Thank...

  11. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...plasmids out of the library in the appropriate combination and do a restriction enzyme digest to cut out...scientists relied on traditional homologous recombination. That technology had been used for decades to...

  12. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...expressed. Pho8Δ60 localizes to the cytosol in an inactive form unless autophagy is induced. Then non-selective... Pho8 from its uncleaved to cleaved form is the final read out of this assay. This is also a quantitative...

  13. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ... DNA that you want to assemble together in a combination of different ways according to your cloning strategy... or PCR products from a gel. You will avoid contamination from other DNA fragments and you will remove...

  16. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...LinkedIn may revoke your ability to send invitations. Finally, if you don’t know clearly why you are inviting...updates, or click bait of any kind. For more on inappropriate content see this article. However, do comment...

  18. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ... (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors to consider...when designing your primers can be found here. Finally, you will want to have a well-organized database...
Showing: 701 - 720 of 929 results