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  1. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...to support your work. For example, if your work requires specialized equipment, try finding institutes ... institutes were able to provide the resources required for him to meet his goals. Therefore, he turned...

  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Similarly, placing UAS upstream of a transgene permits directed expression of that gene in cells that also express...systems, allowing for more complex control over desired gene expression. Take a look at all of the options...

  5. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...cell that gets the plasmid contains all of the desired gRNAs. This increases the chance that all the edits...editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo Lab multiplexed...

  6. Deep Dive: qPCR

    Type
    Blog Post
    ... you to measure the intensity of signal over an entire cycle. Since signals are produced by the presence... practice, multiplexing is quite difficult and requires significant optimization to ensure the results...

  9. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...win a hot chocolate party that will warm up the entire lab. We’ll choose 4 winning submissions and deliver...

  10. CRISPR Guide Update

    Type
    Blog Post
    ...describe newly engineered Cas9s with more flexible requirements and enhanced editing, as well as alternatives...

  12. The 12 Days of CRISPR

    Type
    Blog Post
    ...genome insertion, without DNA cleavage, with CRISPR-directed integrases🎵 On the 6th day of CRISPR, Addgene...

  13. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...creating memories with their labmates! Are you inspired by our 2017 #Deckthelab winners? Be sure to enter...

  14. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...Cultured Warrior (made by Nate using pipette boxes, wire, cable ties) received a festive makeover today✂️...

  17. Addgene at SfN23!

    Type
    Blog Post
    ...tackle any experimental questions you may have. Our Director of Content, Brook, and our scientific curation...

  19. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...ancestral bias in CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest blogger Daniel M. Sapozhnikov...
Showing: 701 - 720 of 906 results