Addgene

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How Addgene Creates Content

Type

Blog Post

...and your research community! We work to provide engaging and informative resources that answer questions...
Plasmids 101: Methylation and Restriction Enzymes

Type

Blog Post

...specialized E. coli strains have been specifically engineered to be Dam and Dcm methylase-deficient, and, as...
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Type

Blog Post

... Besides being fast and efficient, what other challenges does this system help to solve? Myers: Only a...
Plasmids 101: Blue-white Screening

Type

Blog Post

...practical use in the following way. Scientists engineered a multiple cloning site (MCS) into the α-peptide...
Dos & Don'ts When Publishing a Scientific Manuscript

Type

Blog Post

...the journal. Ask colleagues (preferably native English speakers) to proofread your manuscript and help...
Will You Be My Mentor? Finding and Asking for Mentoring Support

Type

Blog Post

...current position Understand your interpersonal challenges if they know some of the people you work with...
Kiran Musunuru on the Newest TALEN Genome-Editing System

Type

Blog Post

...genomes of mammalian or other cells. TALENs are engineered proteins that comprise a DNA binding domain and...
RNA Extraction Without A Kit

Type

Blog Post

...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Type

Blog Post

...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural...
Reproducibility for Everyone: Lessons from an Open Science Collaboration

Type

Blog Post

...working with communities, not for them - Another strength of this collaboration is that it is driven by ...
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Type

Blog Post

...Galvan et al., 2019). The hM4Di receptor is an engineered Gi protein-coupled receptor, one of the Designer...
RaPID Detection of RNA-protein Interactions

Type

Blog Post

...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Type

Blog Post

...structural knowledge of the proteins. To overcome this challenge, the Uppalapati and Woolley lab developed a phage...
Supporting Rare Disease Research with the 2020 BeHEARD Awards

Type

Blog Post

... Empower and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This...
Human Germline Editing Using CRISPR

Type

Blog Post

...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Type

Blog Post

...new plasmids and/or cells or organisms that are engineered can be shared to simplify the comparison by others...
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Type

Blog Post

...~6.5 - 9 *Optimal sequential excitations wavelengths as determined by Rosado et al: see Table 2 for...
Viral Vectors 101: An Introduction to AAV

Type

Blog Post

...and a self-complementary AAV (scAAV) vector. Full-length ssAAV vector genome of both polarities are rescued...
Transferable Skills Guide: Public Speaking

Type

Blog Post

...gives your entire audience a chance to comfortably engage with you. If you can give a seminar, lead a journal...
The time and cost required to make a plasmid

Type

Blog Post

...these rough estimates, it’s clear that community engagement with and use of repositories like Addgene provides...

Showing: 701 - 720 of 802 results

Results per page:

How Addgene Creates Content

Plasmids 101: Methylation and Restriction Enzymes

Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Plasmids 101: Blue-white Screening

Dos & Don'ts When Publishing a Scientific Manuscript

Will You Be My Mentor? Finding and Asking for Mentoring Support

Kiran Musunuru on the Newest TALEN Genome-Editing System

RNA Extraction Without A Kit

Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Reproducibility for Everyone: Lessons from an Open Science Collaboration

Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

RaPID Detection of RNA-protein Interactions

Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Supporting Rare Disease Research with the 2020 BeHEARD Awards

Human Germline Editing Using CRISPR

Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Viral Vectors 101: An Introduction to AAV

Transferable Skills Guide: Public Speaking

The time and cost required to make a plasmid

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