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  1. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...individual diversity to get from gamete to a visibly complex - and unique - adult. My initial research directions...evolutionary perspective brought by studies of comparative anatomy showed me that some of the most fascinating...

  2. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...targets.  Pre-formed primary and secondary antibody complexes (Figure 2B) Some labs have found that you can ...). One issue with this strategy is that large complexes may not permeate through your tissue samples as...

  3. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...several pooled libraries: Cas13X and Cas13Y, two compact Cas13 proteins found in the metagenome datasets...

  4. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...given antibody is available through different companies (Bandrowski et al., 2016). It’s also been well...creating an array of educational materials to accompany our antibody collection.  Addgene is sharing our...

  5. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...epitopes available if it is being selected out of a complex mix of proteins that have been extracted from a...Picking the Right Antibody  It can get even more complex if you’re looking for proteins that are interacting...

  7. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post
    ...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ...secondary structure and high GC content, but some companies now offer Sanger sequencing conditions which can...

  9. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...interest,” Griesbeck said. Because they are chemical compounds, they have to be applied or injected, and they...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...

  11. The MTA at Addgene

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    Blog Post
    ...has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA), which...use for the requested material. Once the RSA is completed, the recipient organization is sent the MTA to...

  12. Visualizing Genomic Loci with CRISPR-Sirius

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    Blog Post
    ...and sgRNA-Sirius-8XMS2 scaffolds performed, they compared them head-to-head against CRISPRainbow. Both tools...visualization tools can be used in live cells. Compared to its predecessor CRISPRainbow, CRISPR-Sirius...

  14. "Build Your Plasmid": The Game - Play to Teach

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    Blog Post
    ...playing a card game called “Magic”. There were complicated rules that governed how this game was played....should be “brains-on”, but as much as I try being compelling, interactive, and application-driven to boost...

  16. With Our New Viral Service, We're Taking CRISPR Further!

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    Blog Post
    ... production of viruses used to deliver CRISPR components to cells or organisms under study. In the past...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...

  18. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction network in... are a few key details. Our set of constructs comprises all SARS-CoV-2 viral proteins except for Nsp3 ...

  19. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Musicophilia is accessible and fascinating, all without compromising on the relevant science."     - Randall Carter...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...

  20. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...link can be your new card. Follow your contact’s company on LinkedIn (or via industry newsletters such as...this because I want it to be clear that it is completely impossible for me or anyone to stay in touch ...
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