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  1. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...After a virion infects a cell, the single particle creates many copies of itself, sometimes up to millions...infected cell.) This is in contrast to bacteria and eukaryotes, which use a binary replication process whereby...

  6. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...again until you don’t.” As every researcher can attest to, science is nothing if not solving your way ...creativity, changed the outcome of the project, or illustrates the value of the work and then build a story ...

  8. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...to the lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for...the cytosol, this ratio is low; but when Keima accumulates in autolysosomes, this ratio is high. When untargeted...

  12. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...KSQ uses a CRISPR-Cas9 platform to systematically test the function of all 20,000 genes in disease models... of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development was...

  13. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...applied for identification of essential MYC binding sites in a wide range of human cells.  A new pooled library...targeting start codons, splice acceptor and donor sites, and introducing premature stop codons.    New...

  15. 10 Great Guest Posts We're Thankful For

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    Blog Post
    ... Dangerous Viruses Contributed by guest blogger Tessa Cressy from the labs of Dr. Elke Mühlberger and ...Medical School describes how AAVs function and indicates important things to consider if you’re getting...

  17. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...reaction requiring luciferin, ATP and oxygen as substrates. Production of photons by this bioluminescent...of Photon Generation Slower Faster Cofactors/Substrates Required Not Required Signal Strength Lower...

  18. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...sequence containing transcription factor binding sites can increase transgene expression and transduction...validate ITR sequences as these enzymes cut at known sites within the ITR. However, small deletions and point...

  19. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ... up on a Saturday at like 7 am to go check your plates and you’re excited to do so, you know that that...Addgene has behind it is something that very much resonates with me.” About Addgene’s role in the future,...
Showing: 661 - 680 of 934 results