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  1. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...can be the best way to get in touch if you have business or follow up. Use the "Contact info" link right...or comment on anything that is not relevant to business or volunteer organizational work. LinkedIn is ...

  5. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... C. elegans embryo when all essential embryonic genes are individually depleted via RNA interference (...that can bind to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In ...

  6. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ..., and as we all know, a structural component of bones and teeth. It is an enzyme co-factor, a crosslinking...with other measurements, specifically fluorescent ones? Many of the available biosensors use a single GFP...

  7. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...Since polyclonal antibodies contain a variety of clones and thus the potential to bind many different sites...secondary antibodies rather than generally reactive ones. And make sure to check for any reported cross-reactivity...

  8. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...genomes, scientists can control expression of their genes of interest. While recombinases are powerful gene... knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the library...

  10. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency between...diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream of light...

  11. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...more I realized that it cuts across multiple disciplines, which really epitomizes what the makerspace ...Caenorhabditis Genetics Center (CGC) at the University of Minnesota has declared our makerspace an educational institution...

  12. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing. The more I talked...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...

  13. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...provides the AAV replication (rep) and capsid (cap) genes. AAV replication uses the host’s polymerase, but... the pHelper plasmid which expresses adenovirus genes which help mediate AAV viral replication; and 3)...

  14. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...can be identified. Even if some mammalian cells lines have similar morphologies, being familiar with these...Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies every 16 hours...

  15. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...proteins both transiently or through stable cell lines. Both methods produce high protein yields if transfection...cells)  that allow for continuous expression of genes integrated into the insect cell genome. Both of ...

  17. Pooled Library Amplifications

    Type
    Blog Post
    ...other colonies containing smaller, recombined backbones that may replicate faster. For this reason, if...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

  18. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...CRISPR plasmids categorized by year of deposit. Outlines show a representative proportion of CRISPR plasmids...of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is both enduring and ...

  19. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools for targeted...nuclease-competent Cas9, obviating the need to generate new cell lines or transgenic animals expressing dCas9 as a means...

  20. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...fewer transcripts of their antibiotic resistance genes and can therefore be cultured in media containing.... Spiking your media with supplements such as magnesium salts, buffering agents, and/or supplying additional...
Showing: 661 - 680 of 902 results