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  3. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...While various Cas9 mutants have been engineered to perform unique editing tasks, classic Cas9 nuclease, which...generating a monoclonal Cas9-expression line prior to performing a screen or even a single gene knockout. Top ...

  4. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...How can I tell if my enzyme will cut? Whether performing a digest for cloning purposes or for diagnostics...consult the REBASE database for more detailed information. Enzyme Dam methylation Dcm methylation EcoKI...

  7. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... genetic system, and a highly versatile DNA transformation system.Unlike most other microorganisms, yeast...plasmids oftentimes utilize an ARS to aid in high transformation efficiency; however, this region does not necessarily...

  8. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At... Step One: Identify the problem and gather information to help you understand it. Step Two: Come up ...

  9. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...interactions with RaPID RaPID is similar to the BioID platform for identifying protein-protein interactions, ...proteins reported by other research groups that performed BirA*-based affinity purification mass spec experiments...

  10. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...similar rates to untreated controls. Ma et al. performed sequencing of 23 potential off-target editing ...advancement in our understanding of how CRISPR will perform in embryos but also shows that many more experiments...

  11. Antibodies 101: Conjugation

    Type
    Blog Post
    ...conjugated antibody will have been verified to perform the same as its unconjugated version, but if you...that you will need to confirm your antibodies’ performance after the reaction.  If you are looking to conjugate...

  12. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...the very nature of the way the experiments were performed, they did not see anything unique to male stem...reporting the sex of cells and tissues—when that information is reliable—is important to ensuring excellence...

  13. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...IIS sites and will be re-digested. In contrast, formation of the desired ligation product is irreversible...enzyme recognition sites after ligation favors formation of the construct of interest. Although efficiency...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    ...time of synthesis and, after a few hours, a conformational change causes the protein to fluoresce red....lentiviral preps in addition to the pooled library format! The library inhibits over 18,000 human genes. ...

  17. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...details below) can also be adapted to a competitive format. Figure 2: Overview of using Competitive...the experiment by diluting your standard curve, performing a serial dilution of your experimental sample...

  19. Hot Plasmids February 2024

    Type
    Blog Post
    ...whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids at Addgene... (Chuapoco et al., 2023). They found the best-performing variant, CAP-Mac, was effective at breaching ...

  20. Engaging with science and society at pgEd

    Type
    Blog Post
    ...crucial if we want to make sure that everyone can be informed and empowered to make their own decisions about...social media and blog posts, lesson plans, or information briefs for policymakers. The rapidly changing...
Showing: 661 - 680 of 963 results