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  2. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ... or have a simple pathology. In these cases, preliminary identification of potential targets by CRISPR...mutations and then test both individual and combinatorial drugs on for effectiveness prior to patient ...

  3. Hot Plasmids - October 2022

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    Blog Post
    ... reliable antibodies. It is derived from the C-terminal region of the human proto-oncogene c-Myc. Since... catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a) antibody is...

  4. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...fused to calmodulin (CaM) and the chicken myosin kinase calmodulin binding region RS20. When calcium is...for single and multi-color imaging experiments. Finally, what kind of system will you use to detect your...

  7. Hot Plasmids and Viral Preps - July 2021

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    Blog Post
    ...controllable gene knockouts, and inducible gene inactivation or activation in an organoid model (Sun, et ...activity.  As SLs are also known to trigger seed germination of parasitic plants, this approach was also used...

  9. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology...types the gene is expressed in and amount of recombinant protein obtained. Selectable Marker The antibiotic...

  10. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ... isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning...Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for protein expression." Current...

  11. Management for Scientists: Managing vs. Leading

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    Blog Post
    ...this quality.   I am often asked to give career seminars on Leadership Skills. After attempting to put ...efficiently and with high quality. Their work involves coordination and can require technical mastery. Scientists...

  12. History of CRISPR Cas - A tale of survival and evolution

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    Blog Post
    ... biotech field, let's look at how prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive...expression of 1 of 5 anti-CRISPR genes were found to inactivate the Type I-F CRISPR/Cas system of Pseudomonas...

  13. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    Blog Post
    ...will help get your experiments back on track.  One final note — this post focuses on the species-on-species... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...

  14. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    Blog Post
    ...– I want to understand my obsession with the combination of chocolate and peanut butter), but it’s an ...Using a programmable microcontroller, NeuroStack coordinates optical stimulation of its associated fibers ...

  15. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post
    ... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...prevent the invading genome from “recovering” its original sequence. If you’re looking to disrupt gene function...

  20. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...roughly) the same field, often through manuscripts, seminars, or posters. It is important to note that it is...does not explicitly exclude people outside of the original recipients. Instead, it targets a specific audience...
Showing: 661 - 680 of 929 results