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  1. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ..., in collaboration with “cloning guru” Kathleen Ottina and David Sabatini at the Whitehead Institute and... over-expressed, constitutively activated or inactivated the pathway. “The idea is that if you introduce...

  2. Editor's Choice, August 2016

    Type
    Blog Post
    ...technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions...

  5. 9 Tips to Achieve Success in Academia

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    Blog Post
    ...than intensely competitive. Be curious and interdisciplinary.   3. “There are many different ways to succeed...science, especially if the conference is a big multidisciplinary one.” Cepko strongly emphasizes that people...

  6. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...philosophy Customer service can be a hassle. You finally make it through a 20-step phone tree, only to be... be left on hold for an hour. When you finally speak to a human being, you may not even get a solution...

  7. Mapping the 4D nucleome with CRISPR/Cas9

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    Blog Post
    ...are thus limited by its lower resolution. The combination of this method with superresolution microscopy... is so high, cells may be imaged under lower illumination settings, lowering photobleaching and phototoxicity...

  10. History of CRISPR Cas - A tale of survival and evolution

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    Blog Post
    ... biotech field, let's look at how prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive...expression of 1 of 5 anti-CRISPR genes were found to inactivate the Type I-F CRISPR/Cas system of Pseudomonas...

  12. CRISPR 101: Drug discovery and CRISPR

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    Blog Post
    ... or have a simple pathology. In these cases, preliminary identification of potential targets by CRISPR...mutations and then test both individual and combinatorial drugs on for effectiveness prior to patient ...

  13. Hot Plasmids - October 2022

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    Blog Post
    ... reliable antibodies. It is derived from the C-terminal region of the human proto-oncogene c-Myc. Since... catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a) antibody is...

  14. Management for Scientists: Managing vs. Leading

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    Blog Post
    ...this quality.   I am often asked to give career seminars on Leadership Skills. After attempting to put ...efficiently and with high quality. Their work involves coordination and can require technical mastery. Scientists...

  16. Plasmids 101: What is a plasmid?

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    Blog Post
    ...Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology...types the gene is expressed in and amount of recombinant protein obtained. Selectable Marker The antibiotic...

  17. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...controllable gene knockouts, and inducible gene inactivation or activation in an organoid model (Sun, et ...activity.  As SLs are also known to trigger seed germination of parasitic plants, this approach was also used...

  19. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...fused to calmodulin (CaM) and the chicken myosin kinase calmodulin binding region RS20. When calcium is...for single and multi-color imaging experiments. Finally, what kind of system will you use to detect your...
Showing: 641 - 660 of 911 results