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Showing: 641 - 660 of 963 results

  2. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...a static measurement and it provides limited information about what materials or organelles are being ... acidic pH found in lysosomes. Therefore, the formation of autophagosomes leads to an increase of yellow...

  5. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...choosing a genomic target. NgAgo also appears to outperform Cas9 in GC-rich regions. DNA guides NgAgo uses...re looking to disrupt gene function via indel formation, this property could be an advantage. However,...

  8. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...but it can be difficult to determine how much information they’ve absorbed if they don’t get a chance apply...gaps in their knowledge. Practically speaking the format I decided on was to have two teams of 4 people ...

  9. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...and the pH shift can be prevented. Keep this information in mind if you’re planning on shipping something...study found that dry ice storage affected the performance of antibodies, because of effects of pH on protein...

  10. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... Web of Science provides basic bibliographic information and uses its vast network of articles to track...including biotech and research tools, computing performance, and randomized control trials in innovation....

  11. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...Renilla luciferase from the sea pansy (Renilla reniformis) emits a blue light with a peak emission at 480nm...Vargulin) luciferin None Yes Sea pansy(Renilla reniformis) 480 36 Coelenterazine None No Deep sea coceopod...

  14. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...different doses. See Figure 4 in this paper for more information.   Q6 & Q7: We have been attempting to transduce...high coverage. Please see this paper for more information about PHP.eB brain labeling at various doses....

  15. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...survive this step. Then, a limiting dilution is performed to isolate individual hybridomas and ELISA is ...Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:258–268. https://doi.org...

  17. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...quite different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell...and mediate efficient viral replication. More information on these viral genes is available in the adenovirus...

  19. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...products for packaging (Gag, Pol, and Env), the isoforms of these proteins, as well as the viral LTRs, ...different viruses and thus use slightly different isoforms of these packaging components. Therefore, lentiviruses...
Showing: 641 - 660 of 963 results