...you would like us to feature in the coming year. Finally, we are always looking for guest bloggers who want...and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter Region – Let's...

...insert. Example, if you have a Flag tag on the N-terminal of your insert, you would list it first. pBACKBONE-Flag-hGene...pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal of your insert you would list it after the insert...

...otherwise be lost amid lab closures or project terminations, ensuring that scientific progress made through... are constrained. This collaborative approach eliminates redundant work, saving time and allowing precious...

... the launch of our fast-growing ready-to-use recombinant antibodies collection, currently composed of ...the community to drive new science experiments. Finally, we were thrilled by how many people accessed our...

...2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute ...cells, deciphering the mechanism of matrix metalloproteinase-9 secretion, and identifying lysine methyltransferase...

...cleavage, the authors showed that Cas14 could indiscriminately cleave ssDNA in trans like Cas13 and Cas12...adding Cas14 to the mix, you get the ultimate combination enabling the detection of RNA, dsDNA, and now...

...forward. Sooner or later enough people will be vaccinated and we’ll have some herd immunity, which raises...Giving think tank event where we brought a multidisciplinary group of scientists together to draft a research...

... timely scientific topics, as well as archived seminar presentations. One of SITN's newest initiatives...The afternoon program mixed things up with a combination of shorter, more narrowly focused lectures, interactive...

...of the high fidelity version (despite what the original paper says). In their hands, BsaI-HF has produced...they hope others will do the same. “This isn’t the final version,” she said. “If we learn how to make the...

...adding plasmid) to make sure you don’t have contamination.  Also conduct a positive control (test a ... Melina Fan...

...technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions...

..., in collaboration with “cloning guru” Kathleen Ottina and David Sabatini at the Whitehead Institute and... over-expressed, constitutively activated or inactivated the pathway. “The idea is that if you introduce...

...different cell types, at different times, and in combination with different genes. Having CRISPR systems for...G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential ...

...that you confirm the details and final experimental outcome in the original publication. As you develop and...

...or in vivo testing. We recommend you check the original paper of your AAV plasmid of choice for more information...be available through our distributor partners in China, Brazil, Japan, Korea, Mexico, and India. Once the...

... more broadly to accelerate COVID-19 research: Sinai Health System                                     ...Essen                      University of North Carolina at Chapel Hill University of Illinois Chicago                     ...

...Addgene ID number as well as the reference for its original publication. To help facilitate this process, ...also increases the visibility of both you and the original depositor. Our citations feature makes it so that...

...philosophy Customer service can be a hassle. You finally make it through a 20-step phone tree, only to be... be left on hold for an hour. When you finally speak to a human being, you may not even get a solution...

...are thus limited by its lower resolution. The combination of this method with superresolution microscopy... is so high, cells may be imaged under lower illumination settings, lowering photobleaching and phototoxicity...

...than intensely competitive. Be curious and interdisciplinary.   3. “There are many different ways to succeed...science, especially if the conference is a big multidisciplinary one.” Cepko strongly emphasizes that people...