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  3. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...targeting efficiency of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells...NHEJ rather than HDR. Figure 1: Homology-directed repair in targeted zygotes proceeds from the maternal...

  5. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...available for a large number of targets, and can require specific conjugates. And, of course, once you have...either re-develop or re-optimize your assay for an entirely new affinity reagent.    Fig. 1: A comparison...

  6. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...and the lab, you may not be able to carry out the entire problem solving process on your own, but you can...skillset is a part of your toolkit is important. Paired with your other skills, both technical and transferable...

  8. Summer SciComm: Preprints

    Type
    Blog Post
    ...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open...’ comments, and author responses can be shared directly alongside the preprint while the work is still...

  9. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...of ccdB, leading to positive identification of desired clones.   ...Becomes a powerful cloning tool... efficiently, as those that do not contain the desired insert should not grow.   Figure 2: Image...

  10. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...those planning a career in academia, it may not be required, but many academic scientists are starting to ...use LinkedIn messaging, not everyone likes to. A direct email can be the best way to get in touch if you...

  12. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...genotype each animal to identify the ones with the desired genotype. Inducible and conditional systems Conditional...conditional knockout mouse, loxP sites are inserted directly into the genome to flank the gene that will be...

  13. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...Therefore, co-infection with a helper adenovirus is required to provide the viral proteins in trans. The Gutless...virus. Purification (2 days) - Purification is required if rAdV is to be used in vivo. The standard method...

  15. Hot Plasmids - October 2020

    Type
    Blog Post
    ... elegans with CRISPR can be difficult since it requires the much less efficient double stranded templates...cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate. Eastman et...

  16. Design Tips for Prime Editing

    Type
    Blog Post
    ...reverse transcriptase (RT) template including the desired edit. Figure 1: Prime Editing schematic....guide Find CRISPR plasmids by function Browse our entire plasmid collection ...

  17. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...functional loss (Ho et al. 2014). To overcome this, paired gRNAs can be used to target specific sizes of a...visualizing ncRNA localization and function, but directly incorporating very long ncRNAs is impractical....

  18. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...antibody affinity: stronger binding antibodies will require harsher stripping to remove, so start with the ...with caution. Unlike PVDF, they can be returned directly to buffer without a re-wetting step. Important...

  19. Hot Plasmids February 2024

    Type
    Blog Post
    ...for your next experiments, they will also likely inspire further-improved versions. Thanks to Christophe...Leterrier, whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids...

  20. 6 Tips for Grant Writing

    Type
    Blog Post
    ...Just like with any sport, hours of practice are required and no one lab or professor becomes an overnight...they are looking for in grant submissions and will direct you on how to submit your work either through seminars...
Showing: 641 - 660 of 906 results