... in signaling processes including smell, taste, sight, inflammation, and neurotransmission. But with approximately...activated downstream of GPCRs that are not involved in sight (Olsen RHJ et al., 2020). A transducer complex consists...Gγ-GFP2 will be in close association, resulting in a high level of GFP2 emission. Following activation in ...

...Chemistry symposium and a career night. The society also visits high schools to promote chemistry degrees...scientific meetings for students to attend. It also hosts high level academic debates for its members to become...

...arrive. Getting off the plane in Bozeman Sunday night, it was easy to spot meeting participants. They ...the ones holding poster tubes (or as our baffled flight attendant called them, "long, skinny things") and...thinking I was an engineer. The talks we heard Monday night represented engineering first and foremost, from...

... allow his team to work mostly at home. Having a high degree of freedom and diversity built into their...new things, diversity of responsibilities, which might include training or teaching others, research, and...sometimes taken to an extreme by scientists) Are highly mobile and can move to a new workplace if opportunities...

...telling it another way. You’ll know you are on the right track if they start asking you questions. Remember...Escape from the Ivory Tower.”  9)   If you think you might want to get serious about science writing, look ...News. Ask your institutional news office if there might be opportunities to blog or write for them. 10)  ...

...bacteriostatic reagents can kill bacteria when used at high concentrations (and vice versa). Looking around ...Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media hotter than about 55oC...55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. ...

... host-pathogen interface, previously developed a high-throughput neutralization assay to study the link...Pfizer or Modern vaccine. This sera was used in the high-throughput SARS-CoV-2 pseudovirus neutralization... KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt AG...

...forward. Here are a few out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring...what’s a scientist to do with no live audience in sight? You could do a talk virtually for your peer mentoring...university or use your current contacts to ask who they might suggest you speak with. See this post that’s all...

...their secondary structure, palindromic nature, and high GC content. This instability can cause partial loss...through ITRs due to their secondary structure and high GC content, but some companies now offer Sanger ...example, CAG is a strong promoter that drives very high, ubiquitous expression, while the human synapsin...

...Baradaran says.  Baradaran’s path to science began in high school. “I had a great chemistry teacher in 10th...medicines can have on human health. As a teenager in high school, I knew that this is what I wanted to do,...ClonTracer Barcoding Library, a library that “enables the high-resolution tracking of more than 1 million cancer...

...and it is under copyright, you will need to obtain written approval from the copyright owner and will ...treatment conditions like drugs (Chemogenetics) or light (Optogenetics) to activate receptors? If you’re ...

...the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors, and microfluidic...channels. These features enable the fibers to emit light for optogenetic purposes, measure temperature, study...construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the...

...scientists, MTAs are important—they guarantee certain rights and protections for depositor and requestor alike... must be executed. The Golden Stopwatch Award highlights TTOs that accelerate science by processing agreements...

...Keima accumulates in autolysosomes, this ratio is high. When untargeted, Keima can be used to measure bulk...autophagy-related microtubule-associated proteins 1A/1B light chain 3B, or LC3. LC3 is a commonly used marker ...oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources on Addgene.org...

...Delivery The RNP delivery method consistently leads to high on-target editing and low off-target editing rates...SpCas9 The closer your edit is to the PAM site, the higher the efficiency of HDR repair. Check out this post...post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative...

...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually... <- Can move between the two (helps to know the right people) -> How can you make a decision about ...interests and passions is another great way to shed light on what paths to pursue. Spend some time really ...

...’d like to show our gratitude on the blog by highlighting some of the great posts written by Addgene depositors...to activate neurons and neuronal pathways using light and chemicals respectively. Didem Goz Aytruk from...

...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...science storytelling. You can introduce your results right at the beginning of your communication, use conflict...storytelling options, and they all can be effective in the right circumstances.   However, in the midst of all these...

...pBR322, pACYC, and pUC were developed to provide higher copy number vectors that could be used in these...updated and added new content to our eBook. Some highlights of the 2nd edition eBook include: new chapters...

...but thought we could learn a little bit more about high throughput DNA prepping by trying out NEB’s new ...generating a wide range of insert sizes and also produced high yields of quality libraries from small amounts of...summarize my experience in my first blog post! I highly recommend doing the same if you ever go to a workshop...