...Building Community.” This post leads readers to our latest podcast all about graduate student life and how...myself and two of my previous graduate school classmates, Ben Vincent and Niroshi Senaratne. When we recorded...

...as CRISPR-based gene repression tools to find and test genes that may play a role in the development of...polymerases produced using plasmids from Addgene to test for the presence of pathogens in environmental samples...

...are used to insert recombinant DNA from prokaryotes or eukaryotes into a synthetic vector that can have ...

...demonstrate efficient editing in adult mice and in zygotes. Read the paper in Molecular Cell Find the Nme2Cas9... was developed by Rebecca Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, ...

...2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute ...secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR v2 is...

... This blog was originally published on BitesizeBio here. As part of my job ensuring plasmid quality at...Verify Plasmids Acknowledgements: Thank you BitesizeBio for originally publishing this and allowing us...

...compensation committee. The compensation committee creates a compensation philosophy that helps guide employee...spend time caring for my mother. For fun, I enjoy pilates, jewelry making, reading, sailing, and gardening...

...be repurposed for double strand breaks at target sites. Read the paper in Nature Communications Find...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one...

...And could it be a new tool for genome editing? To test the potential of Cas14, the Doudna lab cloned the...of dsDNA and RNA in trans. Fluorescent signal indicates the presence of this viral sequence in the assay...

...nature of the Center for Regenerative Medicine accelerates research. Chapter 5: Research currently under... defines Open Science and how he practices and promotes open science in the lab - sharing reagents, cell...

...transfers. The UBMTA was drafted by the National Institutes of Health (NIH) and adopted by the Association... Processing: When you place an order, Addgene generates the MTA and emails it straight to you, or we can...

...being plated on agar with ampicillin selection.  Notes and tips  We’ve found that our competent cells ...contamination.  Also conduct a positive control (test a plasmid that transforms well in other competent...

...Michael Koeris to learn how his company uses phage to test for Listeria in food products). I picked this post...Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific Enterprise in ...

...might have a few positive control genes on which to test the validity of your biological model and the technical...starting point for developing a model and an assay to test that model. Both libraries are available from Addgene...

...Given the variety of these enzymes and the unique sites they recognize, restriction digests have become ...cleave DNA at specific sequences called restrictions sites. Often, the size of the plasmid insert and vector...Analyzer , allow you to identify which restriction sites are present in a given sequence. For a list of the...either PCR - or restriction enzyme -based cloning to test individual clones before use of more expensive forms... restriction enzymes common to multiple cloning sites, will result in similar sized bands, thus making...

...which labs should receive the clinical sample to test their phages. Because the sample is limited, only...highest potential to possess the correct phages will test. These includes labs with a large number of different...

...save you the time that would be spent making and testing entirely new gRNA designs. You can now easily find...variations (e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus to ensure...

...isolation of successful transformants. Addgene distributes several ready-to-use, modular plasmids, combining...combining fluorescent tags, epitope tags, protease sites, and selection markers. These are especially useful...

...new window) Link to Video Making LB Agar Plates Create plates to culture bacteria in the lab Watch the...molecular biology, plasmid cloning, and titering and testing your viral preparations. Addgene...your plasmids. Virus Protocols for titering and testing your virus preparations. Antibodies Protocols for...and ligation Cloning by PCR Generate restriction sites by PCR Modification by Annealed Oligo Cloning Add...

...research by anyone. As infection rates were increasing in the United States, and many universities and Technology...