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  2. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...transduction methods. Some ‘difficult to transfect’ cells lines are amenable to transduction, making it a logical...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene...

  3. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post
    ... and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like...certain guide sequences - using 5 guides/gene for 10 genes, they did not observe differences in cleavage efficiency...

  5. 25 Work From Home Tips for Scientists

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    Blog Post
    ... help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice...19. You can also give back by supporting local businesses (ex: purchasing a gift card to use later or ordering...

  6. Visualizing Genomic Loci with CRISPR-Sirius

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    Blog Post
    ...the gRNA target site. In addition to increased brightness, they also found that the signal to noise ratio... observing low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly,...

  8. Trading Spaces - Visiting Scientist Edition

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    Blog Post
    ... know that science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for...applying to a structured program, they likely have guidelines to consult on this topic.   Etiquette...

  9. K. phaffii: Rising to the Occasion

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    Blog Post
    ...experimental model. In fact, approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

  11. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...performed using traditional 2D assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in .... P., Herskovitz, J., Goletz, T. J., Zhou, L., Moxness, M., Flynn, G. C., Narhi, L. O., & Jawa, V. (2016...

  12. Cultivating Community Science at BosLab

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    Blog Post
    ... with audiovisual media, software engineering, business consulting, mathematics, and more. That’s right...BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge with the community...

  13. Networking Follow-up: I Met a Person. Now What?

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    Blog Post
    ...my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn profile...She supported me and answered my questions and honestly, she was the only person who really understood...

  14. What Good Citizenship Can Do for Reproducibility in Science

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    Blog Post
    ...going forward. This is not just a question of being honest and straightforward, it’s an important part of ... various different versions of checklists and guidelines to help authors understand what they are expected...

  15. 5 Great Apps for Lab Life

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    Blog Post
    ...smartphone to help me with my research in terms of preparedness, organization, and sharing data. Here are five...Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State University. He works with gene expression...

  16. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...disrupt target gene sequences (thus inactivating genes), while breaks repaired by homology directed repair... which sequence replacement holds some kind of fitness advantage but error-prone repair of the gene would...

  17. Components of CRISPR/Cas9

    Type
    Blog Post
    ...Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...sites with the aid of two RNAs: the crRNA which defines the genomic target for Cas9, and the tracrRNA which...

  18. New and Upcoming Viral Vectors - May 2020

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    Blog Post
    ...inhibition library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening... of the extensive supply of transgenic reporter lines that supply a variety of GFP expression patterns...

  19. Early Career Researcher Toolbox: Social Media for Scientists

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    Blog Post
    ...interviews on Al Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences...be wondering how to get started. This blog post outlines 10 steps to take when starting to use Twitter ...
Showing: 621 - 640 of 902 results