...you realize whether or not you have a natural inclination for this skill that you could strengthen. The...substantive (“Person C told Person A one thing and inadvertently told Person B something else.") The majority...through multiple channels is not reasonable or sustainable. That said, when you know that a person or team...

... scFvs. What is an scFv? scFVs are a type of recombinant antibody. They are ~25 kDa single polypeptides...from the B cell antibody genes from diseased or vaccinated humans. How do you produce scFvs? While it’s ...coli Expression System in Producing Antibody Recombinant Fragments. IJMS 21:6324 . https://doi.org/10.3390...

...to have a check-in with my boss Jennifer. I had finally found my footing with the company. I’d identified... Bench series about PPE! We made this with a combination of in-lab filming and remote filming. Learn: ...ensure smooth filming in the future, but we were finally refining the process down to a place I could be...

...career scientists are those in the Young Scientist Seminar Series. Watch how these speakers talk without looking...their level of explanation in presenting data. Finally, let me take this opportunity to recommend all ...your slides for typos and clarity in a big room.  Finally, don’t pass up opportunities to show cool visuals...

...individuals. Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving life expectancy and quality... designed multiple gRNAs to target the 5’ long terminal repeat (LTR) of the HIV-1 genome, which acts as... Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination of HIV-1 Genomes from Human T-lymphoid Cells ...

...While off-target effects are nearly impossible to eliminate, it’s essential to understand them in order to...repair to allow for detection, not Homologous Recombination or Alternative End Joining, two alternative ...different gRNA would have distinct off-target sites, eliminating its effectiveness as a control. Since off-targets...

...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although the lab used this...thought to be reversible once the effector is inactivated/removed from the system. In contrast, epigenetic...

...transformation due to background re-circularization and contamination from uncut plasmid. 4. Transformation Transform... higher competency cells. Additionally, if your final product is going to be very large (>10kb) you might...DNA and lets other materials pass through; and, finally, selectively elute the plasmid DNA from the column...

...be analyzed with a paired t-test, because they eliminate subject-to-subject variation (the test assesses...tests exist to identify significant pairwise combinations – Tukey’s test, Newman-Keuls, Duncan’s multiple...Comparison of Multivariate Means across Groups with Ordinal Dependent Variables: A Monte Carlo Simulation Study...

...of dsRNA. In plants, CRISPR technologies are predominantly used to disrupt function at the level of DNA...Darren Gilmour at the University of Zurich. He is fascinated by how organisms exquisitely and robustly self-organize...Interference System in Physcomitrella patens Reveals a Dominant Role of Augmin in Phragmoplast Microtubule Generation...

...antibodies here. Recombinant antibodies Similar to monoclonal antibodies, recombinant antibodies are a...from naturally-occurring immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences...

...14] Hepatocytes [15] Pancreatic β-cells [16] Intestinal tissue [17] Lung epithelial cells [18] Red ...specific to the lineage of the target cell in the original somatic cells [41]. The in vivo and in vitro methods...differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature, 2011. 470(7332): p. ...

...collection of IPI’s well-validated and ready-to-use recombinant antibodies. The first collection, a set of integrin...sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access to IPI...

... and quantify how new technologies are being disseminated through the scientific community. Data on CRISPR...technology was also greatly accelerated through a combination of open-source distributors such as Addgene, ...

...CapsidsResearchers: Cynthia Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation... Gut to BrainResearchers: Atharva Sahasrabudhe; Polina Aniveeka   A big thank you to all who have contributed...

...on the Addgene repository, are also attending! Finally, our team is rounded out with our science communication...conference experience on X, Instagram, and Bluesky.   Finally, if you're looking to brush up on your conference...

...It’s finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing...or lab manager’s approval before letting your imagination run loose on the lab. Have fun! Here’s an example...

...Originally published May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp...opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert adenosine to inosine. Activate/Repress...

...offering an AAV2 CAG-driven tTA expresser from the Gradinaru lab. This is useful for both in vitro and in vivo...serotypes pAAV-CAG-tTA AAV2 Molecular Tools Gradinaru New viral service pAAV_hSyn1_nLightG AAV9 Biosensors...

...we wouldn’t necessarily have expected when we originally sent out the call for applications. Cervical ...caused by persistent infection of HR HPV types, predominantly HPV 16 and 18.” HPV plasmids as positive controls...