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We narrowed to 906 results for: IRE

Showing: 621 - 640 of 906 results

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Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Type

Blog Post

...nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to recognize the target. Osamu Nureki...such as transcription factors tethered to dCas9, require long time scales with constitutively expressed ...
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

Type

Blog Post

...serotonin. Using a combination of computer modeling, directed evolution, site-saturation mutagenesis, and medium-throughput...anxiety, but these drugs are often ineffective or require several rounds of dose and drug adjustments. Unger...
Using CRISPR/Cas9 to Edit Disease Out of the Genome

Type

Blog Post

...Berkeley’s Jennifer Doudna. “They are the first direct demonstration of use of genome editing to correct...need to get a handle on how cleavage sites are repaired.” Those insights will be critical for scientists...
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

Type

Blog Post

... research projects to a halt. None of us works directly with viruses or transmissible diseases, so we ...and availability; 3) Make the contact to start a direct collaboration between researcher and expert; 4)...
Multiplexed Overexpression of Regulatory Factors (MORF) collection

Type

Blog Post

... presence influences cell fates. The atlas of directed differentiation To understand how each human ...Joung J. et al., A transcription factor atlas of directed differentiation. Cell. 2023. 186 (1) 209-229. ...
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

Type

Blog Post

...tissue types! Although putting a gene of interest directly downstream of a tissue-specific promoter sounds...34, 1–15 (2002).PubMed PMID: 12324939. 3. S. E. McGuire, G. Roman, R. L. Davis, Gene expression systems...
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

Type

Blog Post

...illuminated with green light. They found that KillerRed required longer exposure times (5 minutes vs 10 seconds...longer exposure times. Additionally, KillerRed requires mitochondrial targeting to increase cell ablation...
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

Type

Blog Post

...DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying...editing target site on the DNA, the Cas domain directs the modifying enzyme to the target site, and the...
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

Type

Blog Post

...the research is ongoing, but all registries do require that research plans become public eventually). ...implement authentic scientific research and as the Director of Advising in the Division of Life Sciences at...
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Type

Blog Post

...limited deep-tissue performance, and sometimes require an alternative such as a radioactive tracer. As...cell differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9...
Building and Validating Recombinant Antibodies: A Case Study

Type

Blog Post

...to WGS the lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation...
Antibodies 101: Isotypes

Type

Blog Post

...response, the variable region of an antibody is paired with any of the other isotype heavy chain constant...deleted. Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. ...
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

Type

Blog Post

...thus HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target...or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown. Multiplexing...
From Lab Bench to Leadership: Transitioning into Management as a PhD

Type

Blog Post

... goals, and fostering team success. This shift requires deliberate preparation and a willingness to embrace... members thrived on independence, while others required more guidance and structure. I quickly learned...
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

Type

Blog Post

...same chromosome. Targeted Insertion allows for direct comparisons between lines expressing different ...wondering, “How do I know which of my flies have the desired integration?” Not to worry! In flies, phenotypic...
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

Type

Blog Post

... contributed by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad Institute of...Jess S. Boehm. Jesse S. Boehm is the Associate Director of the Cancer Program at the Broad Institute of...
Sharing is Caring: But How to Distribute Open Hardware?

Type

Blog Post

...clarified, especially with regard to licensing models. Direct sharing of work from universities and research ...Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying ...
GPCRs: How Do They Work and How Do We Study Them?

Type

Blog Post

...new ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth...chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural ligands. These...
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Type

Blog Post

...antibodies to recognize and bind transcription factors directly, but that’s a slow-going process and it doesn’...transcription factors. The second vector is a homology directed repair donor plasmid with homology arms and a ...
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Type

Blog Post

...screens that can be done with the libraries will require access to next generation sequencing technology...due to the size of the backbone, transformations require electroporation. References & Further Reading...

Showing: 621 - 640 of 906 results

Results per page:

Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

Using CRISPR/Cas9 to Edit Disease Out of the Genome

Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

Multiplexed Overexpression of Regulatory Factors (MORF) collection

Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Building and Validating Recombinant Antibodies: A Case Study

Antibodies 101: Isotypes

Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

From Lab Bench to Leadership: Transitioning into Management as a PhD

Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

Sharing is Caring: But How to Distribute Open Hardware?

GPCRs: How Do They Work and How Do We Study Them?

Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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