...Aqua Cyan Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian...pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene...pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian Express a gene...

...relating to reproducing the original research can be documented -- in the original context in which it was...import Word documents into RSpace, maintaining the original formatting. In this way data is moved from a static...

...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired... to I Replacement) system fuses Cas13b to RNA deaminase ADAR2 to create a specific RNA editor. Cas13 enzymes...JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR (2018) Evolved...

...indicates that the cancer has spread from where it originated to another organ in the body. Such misdiagnosis...mesothelioma tumors from the peritoneum and any other abdominal organs to which it may have spread. Then, the ...performs a heated chemotherapy wash throughout the abdominal cavity. This targeted chemotherapy approach has...

...resistance. Source: https://www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will very likely...develop over time. It isn’t feasible to completely eliminate the development of resistance; it’s a natural .... "Tackling drug-resistant infections globally: final report and recommendations." London: Wellcome Trust...

...Science subreddit is well moderated. One of our original worries about sharing science advice on Reddit...is abundant, helpful, and sometimes excellent. Finally, as I said in the AMA, it’s incredibly important...find my tips for a perfect science talk here). Finally, my main content organizer is the amazing app Evernote...

...marketing, commercialization, collaborative, and financial functions associated with the business. Commercial...business, in healthcare economics, or in some combination of these fields. I got my PhD a couple of years...trends and workings of the healthcare industry. Finally, on-campus resources like career offices, classes...

...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although the lab used this...thought to be reversible once the effector is inactivated/removed from the system. In contrast, epigenetic...

... scFvs. What is an scFv? scFVs are a type of recombinant antibody. They are ~25 kDa single polypeptides...from the B cell antibody genes from diseased or vaccinated humans. How do you produce scFvs? While it’s ...coli Expression System in Producing Antibody Recombinant Fragments. IJMS 21:6324 . https://doi.org/10.3390...

...to have a check-in with my boss Jennifer. I had finally found my footing with the company. I’d identified... Bench series about PPE! We made this with a combination of in-lab filming and remote filming. Learn: ...ensure smooth filming in the future, but we were finally refining the process down to a place I could be...

...blotting, a primary epitope-targeting antibody can originate from a range of species; for example, rabbit or...include cytokines and chemokines; enzymes like kinases, phosphatases, and ubiquitin ligases; and many ...  More resources on Addgene.org Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide...

...the literature was reproducible by their groups. Finally results from the Reproducibility Project: Cancer...to the daily news briefing, to quick notes, to finances, to flights & ordering food - everything is covered...significant barrier to ELN adoption. This includes financial outlay, staff hours, troubleshooting, and the ...

...you realize whether or not you have a natural inclination for this skill that you could strengthen. The...substantive (“Person C told Person A one thing and inadvertently told Person B something else.") The majority...through multiple channels is not reasonable or sustainable. That said, when you know that a person or team...

...sequence, people often use terminology like “C-terminal fusion,” but I find this very confusing because... into the POI can work well since the N- and C-terminal residues of the fluorescent protein are relatively...assess whether the chimera is still functional. Final words on fluorescent protein fusions There are several...

...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...cloning of your gRNA of interest. In recent years, deaminase-mediated base editing (cytosine base editor or...FJ, Fan C, Landry MP (2019) DNA nanostructures coordinate gene silencing in mature plants. Proc Natl Acad...

...career scientists are those in the Young Scientist Seminar Series. Watch how these speakers talk without looking...their level of explanation in presenting data. Finally, let me take this opportunity to recommend all ...your slides for typos and clarity in a big room.  Finally, don’t pass up opportunities to show cool visuals...

...individuals. Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving life expectancy and quality... designed multiple gRNAs to target the 5’ long terminal repeat (LTR) of the HIV-1 genome, which acts as... Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination of HIV-1 Genomes from Human T-lymphoid Cells ...

...transformation due to background re-circularization and contamination from uncut plasmid. 4. Transformation Transform... higher competency cells. Additionally, if your final product is going to be very large (>10kb) you might...DNA and lets other materials pass through; and, finally, selectively elute the plasmid DNA from the column...

...While off-target effects are nearly impossible to eliminate, it’s essential to understand them in order to...repair to allow for detection, not Homologous Recombination or Alternative End Joining, two alternative ...different gRNA would have distinct off-target sites, eliminating its effectiveness as a control. Since off-targets...

...of dsRNA. In plants, CRISPR technologies are predominantly used to disrupt function at the level of DNA...Darren Gilmour at the University of Zurich. He is fascinated by how organisms exquisitely and robustly self-organize...Interference System in Physcomitrella patens Reveals a Dominant Role of Augmin in Phragmoplast Microtubule Generation...