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We narrowed to 942 results for: OMP;

Showing: 601 - 620 of 942 results

  1. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...However, one of the many proteins which are not compatible with regular mesophilic systems are the psychrophilic...chaperones protein resulted in higher levels of growth compared to the E. coli strain expressing the empty vector... Medal in the Undergrad category at the iGEM Competition 2019. He has an active interest in scientific...

  2. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...to start a more formal mentoring program at my company, but there weren't enough senior people willing... such as pre-reads, videos or exercises to be completed in preparation for the meeting or to be read or... and help each other practice meeting people – compete for fastest to set up a coffee date or most cards...

  3. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... light sensitive and have enhanced conductance compared to current channelrhodopsins. Their new channelrhodopsins...neurons do not express as many copies of the ChRs as compared to direct injection. This lower multiplicity of...better suited for systemic delivery with PHP.eB compared to the existing ChR2(H134R).  In the second experiment...

  4. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it can also detect...without fixation or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed...bacteriophage shell protein or a bacterial microcompartment protein. The expression of this fusion is ...

  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it will proceed to ...that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged by its target sequence. Why...Cas7-11 Cas7-11 is a naturally occurring RNase composed of the fusion of several Cas7 and Cas11 domains...

  6. To Each HIS Own

    Type
    Blog Post
    ...proteins within a system. This can be pretty complicated given that a single cell has tens of thousands...strategy a single protein can be isolated from a complex mix using affinity chromatography against an amino...then eluted using a high imidazole buffer that outcompetes the interaction between the protein of interest...

  7. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ... had been collecting data and documenting the compliance of universities with the FLSA in our online resource... salary raising as the only viable option for compliance. However, 10 days prior to the implementation...opportunity of the lapsed mandate (or are being compelled at the state level, in the case of some public...

  8. RNA Extraction Without A Kit

    Type
    Blog Post
    ...or tissue collection method, so biotechnology companies have marketed several products that help to further... RNAzol®, QIAzol®  and sold by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol... is the carryover of DNA that can potentially complicate results of a downstream application such as quantitative...

  9. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ... was also between 1013 - 1014 vg/kg, a dosage comparable to those currently used in human gene therapy...fluorescent voltage indicators, however, fall short compared to GFP-based sensors in terms of response and ...precision of gene expression and reduces toxicity as compared to previous Cre/lox or drug-induced Cre systems...

  10. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...Stanford University. Sex and gender are critical components of biological research that are often forgotten...cost at this early stage of research is minimal compared to that of human clinical trials. And, as Janine...studies that draw from a body of research that is incomplete (because sex was not analyzed) may harm humans...

  11. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...Cloner, and Snapgene), and save these to a shared computer or server. This will ensure that future members...more than two sequencing reactions. In the more complex cases, we sometimes have to design new primers ...saves us the step of purifying the DNA and gives comparable success rates and sequence quality, which is ...

  12. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...itself. Among other things, science communication comprises broadcasting, science writing, and certain aspects... get on stage for a Science Slam – a sort of competitive TED talk – to win the audience’s applause with...photography and participate in a science photo competition. Or nominate yourself as an educator for a short...

  13. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...get the molecular components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA complex) and the donor ...destabilizes the cell’s outer membrane and allows the components to physically enter the cells so they can go ...

  14. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...difficult to functionally manipulate and study in comparison to protein-coding genes (Rinn and Chang 2012)...deletions and insertions in the DNA sequence can completely disrupt expression of the transcribed ncRNA (...interactions between ncRNAs and adjacent genes are complex and not yet fully understood, so it is important...

  15. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...machinery.   Well, I’ve got some good news: complete DNA demethylation can be achieved by dCas9 alone...too.  High dCas9 and gRNA expression are key for complete DNA demethylation – tight binding is crucial for...Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands...

  16. CRISPR in the Clinic

    Type
    Blog Post
    ...well as the introduction of the cancer-fighting component, CAR. Clinical trials are underway to explore ... have involved turning on fetal hemoglobin to compensate for defects in the adult hemoglobin produced ...for use in the next few months. Several other companies have followed suite, with Beam Therapeutics initiating...

  17. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...choosing antibodies   Complexity is a key factor Biological systems tend to be complex with a variety of ...proteins interacting. Experiments tend to be equally complex with users trying to probe multiple targets in ...

  19. Engaging with science and society at pgEd

    Type
    Blog Post
    ...of science, even while I was a bench scientist completing my graduate studies in molecular biology and ...for the past three years. pgEd is a small team comprised of individuals from diverse backgrounds and disciplines...social context. This “general public” audience comprises the taxpayers who fund much of scientific research...

  20. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...response against AdV. Importantly, recombination-competent adenovirus (RCA) can be generated if the E1 gene...negligible toxicity. However, their production is more complex and the presence of helper virus contaminants have...amplification.*Pro-Tip*: The cells will become completely confluent and the media will turn yellow. Do ...
Showing: 601 - 620 of 942 results