...virulent or antibiotic resistant bacteria, and inappropriate antibiotic usage selects for resistant bacteria...including Chipotle and Panera Bread have pledged to eliminate the use of antibiotics by their suppliers, and... DSB occurs on a plasmid, the plasmid will be eliminated from the bacterium, which may also induce cell...

...signal sequence (gene start) and a transcription termination/polyadenylation signal sequence (gene end). A...encoded in separate (and thus smaller) plasmids. Finally, having four separate plasmids allows researchers...it reaches the gene end signal, where it will terminate transcription and polyadenylate the mRNA. These...

...isoforms of these proteins, as well as the viral long terminal repeats (LTRs) are different. As a result, lentiviral...modified the 5’ LTR of the transfer plasmid to eliminate the need for the HIV Tat protein and consequently...initial infection. SIN is shorthand for self-inactivating, which is achieved by deleting a large portion...

...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired... to I Replacement) system fuses Cas13b to RNA deaminase ADAR2 to create a specific RNA editor. Cas13 enzymes...JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR (2018) Evolved...

...mice is likely due to how the Deverman and the Gradinaru labs engineered PHP.B. PHP.B was created by first...suggested there was a single gene that had two codominant versions or alleles that was driving the PHP.... available from Addgene. References Holmes, Christina, and William L. Stanford. "Concise review: stem...

...-dead variant of Cas9 (dCas9) and engineered combinations of transcriptional activators to induce gene...its expression. Constitutive or inducible Cre recombinases can be introduced by AAV or by mating with Cre-expressing...neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin...

...relating to reproducing the original research can be documented -- in the original context in which it was...import Word documents into RSpace, maintaining the original formatting. In this way data is moved from a static...

...strongly supported their initial findings.  The team originally suspected that this new Cas7-11 might be phylogenetically...flp didn’t work – it turns out these tyrosine recombinases are as bad at integrating as they are good at...Christopher A. Vakulskas, John A. Walker II, Anastasia P. Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. ...

...Addgene, you’ll usually receive an aliquot from the original library constructed by the depositing lab. (For... library should be very similar to that of the original library. Like amplification, this step also depends...concentration.  Screening time Phew! You are now (finally!) ready to use your transduced cells to perform...

...methods that do not involve modification of the original genome by integrating viral vectors are preferred...PubMed Central PMCID: PMC3193421. 4. Hacein-Bey-Abina, S., et al., LMO2-associated clonal T cell proliferation...small amount of human peripheral blood using a combination of activated T cells and Sendai virus. Nat Protoc...

... full plasmid sequencing at our facility using Illumina MiSeq Next Generation Sequencing (NGS) technology...sequences each week. Our internal software was originally designed to help us work in large formats, such...continuous updates. The generous donation of a Illumina MiSeq sequencer enabled us to perform the necessary...

...indicates that the cancer has spread from where it originated to another organ in the body. Such misdiagnosis...mesothelioma tumors from the peritoneum and any other abdominal organs to which it may have spread. Then, the ...performs a heated chemotherapy wash throughout the abdominal cavity. This targeted chemotherapy approach has...

...resistance. Source: https://www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will very likely...develop over time. It isn’t feasible to completely eliminate the development of resistance; it’s a natural .... "Tackling drug-resistant infections globally: final report and recommendations." London: Wellcome Trust...

...Science subreddit is well moderated. One of our original worries about sharing science advice on Reddit...is abundant, helpful, and sometimes excellent. Finally, as I said in the AMA, it’s incredibly important...find my tips for a perfect science talk here). Finally, my main content organizer is the amazing app Evernote...

...marketing, commercialization, collaborative, and financial functions associated with the business. Commercial...business, in healthcare economics, or in some combination of these fields. I got my PhD a couple of years...trends and workings of the healthcare industry. Finally, on-campus resources like career offices, classes...

...Aqua Cyan Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian...pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene...pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian Express a gene...

...sequence, people often use terminology like “C-terminal fusion,” but I find this very confusing because... into the POI can work well since the N- and C-terminal residues of the fluorescent protein are relatively...assess whether the chimera is still functional. Final words on fluorescent protein fusions There are several...

...you realize whether or not you have a natural inclination for this skill that you could strengthen. The...substantive (“Person C told Person A one thing and inadvertently told Person B something else.") The majority...through multiple channels is not reasonable or sustainable. That said, when you know that a person or team...

... scFvs. What is an scFv? scFVs are a type of recombinant antibody. They are ~25 kDa single polypeptides...from the B cell antibody genes from diseased or vaccinated humans. How do you produce scFvs? While it’s ...coli Expression System in Producing Antibody Recombinant Fragments. IJMS 21:6324 . https://doi.org/10.3390...

...to have a check-in with my boss Jennifer. I had finally found my footing with the company. I’d identified... Bench series about PPE! We made this with a combination of in-lab filming and remote filming. Learn: ...ensure smooth filming in the future, but we were finally refining the process down to a place I could be...