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  1. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...widely adopted. So thank you to the camelids who inspired this versatile family of tools, the applications...Antibodies  Secondary Nanobody Toolbox No Llamas Required - Synthetic Nanobodies Against Membrane Proteins...

  2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...Your next cool experiment requires some AAV.  Where do you start? Plasmids of course! You just need three...gene The rep gene encodes four proteins that are required for viral genome replication and packaging: Rep78...

  4. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...2 components: 1) an SB transposase, the enzyme required for catalysis of transposition; and 2) a transposon...Beauty transposase as a nonviral vector for CD19-directed chimeric antigen receptor (CAR) therapy. SB is...

  6. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...over the sides.  These tiny gels and tiny lanes required less agar, less ethidium bromide, less stop buffer...commercial boxes were small enough to minimize the required area and buffer volume.  So, I designed my own...

  10. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...large amounts of the target protein are typically required due to the relatively low efficiency of biotinylation... version 2.0? Instruments such as an Octet may require less in vivo-biotinylated protein (total amount...

  11. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...optimized for your application. Depending on the tag, directly imaging or tracking your protein may also be possible...is not ideal with Myc, as low pH conditions are required during the purification, which may affect protein...

  13. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...varies. See below for additional considerations. *Requires pir gene for replication (reference). **F1 is...always pick a single colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures...

  15. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...down viral genomes containing only the sequences required for viral replication and transcription as well...binding proteins, dCas9, and a modified gRNA to direct a variety of customizable functions to your DNA...

  16. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...yourself, you can now order ready-to-use virus directly from Addgene! As part of our new Viral Service...Virus production can be a frustrating process that requires a large investment of time and resources for labs...

  17. Plasmid Cloning by PCR

    Type
    Blog Post
    ...: Do not cut within your insert Are in the desired location in your recipient plasmid (usually in ...amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying from a plasmid or...
Showing: 601 - 620 of 906 results