...donor, you can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This...provided you have some idea of where in the genome you might expect off target editing to occur. Luckily, in ...you to Sanger sequence a manageable number of the higher probability locations. If you’re worried about ...Bombieri N, Giugno R, Pinello L. CRISPRitz: rapid, high-throughput and variant-aware in silico off-target...

...to make them serve you well. The happy byproduct might just be a career opportunity. The focus of this...content. Create a Google.slides or Powerpoint file right now to capture interesting key results, striking...need to define a term, but are worried that this might sound condescending, consider explaining the term...Additional Resources Joanne's "How to Choose the Right Next Lab or Workplace" On YouTube Joanne's 10 Things...

... from a default OFF to an ON state, and how you might use these in your research. Today, we’ll look at...Take a look at all of the options and decide what might work best for your experiments! This post was originally...References and Resources References Gossen M, Bujard H. Tight control of gene expression in mammalian cells by...PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection of VP16, the trans-activator...

... site.  T-RL= transposon right-left orientation. T-LR= transposons left-right orientation. Image from ...2009). hySB100x improves on SB100X by having 30% higher transposition activity (Voigt et al., 2016). Sleeping...sequence into the zygote’s genome, a process that’s highly unpredictable. Transposons, however, efficiently...

...backwards. To publish a submitted manuscript in a high-profile journal typically takes 6-12 months depending...technology may not exist to answer the question or it might be extremely difficult for you to get the needed...for a summer undergrad, rotating grad student or high school student. Learn to teach how to design, perform...improves your perspective like intense exercise. I highly recommend running or biking or something that demands...

...activated, the protein emits a brief yet bright burst of light, visible to the naked eye, at regular intervals...forever change the fly lab experience. The Frequency Light Interval System, or FLIS, incorporates a bioluminescence...

...Addgene we couldn’t be more excited! As a small, tight-knit organization we thrive on 3 things: fun, teamwork...trophy among 6 people, but you’ve earned bragging rights for the next 12 months. Your team’s creativity,... favorite lunch... a BonMe noodle meal, crammed right into the box. Which is your favorite team costume...

...service pAAV_hSyn1_nLightG AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors...of synapses distant to the site of injection with high temporal precision.   Table 1: New ready-to-use...

...little screening for DNA markers like the presence of high risk (HR) Human Papillomavirus (HPV). As Gladys ...individuals who are infected with HR HPV and therefore at high risk for cervical cancer development and also better...disease severity. Individuals who are shown to be at high risk by these screens can then undergo more routine...

...targeted destruction.  Antibodies are composed of two light chains and two heavy chains. The ends of the branches...branches contain the antigen binding sites and are highly variable, allowing exquisite specificity. This ...rabbit antibodies that exhibit increased avidity to high profile antigens; and 4) generating mAbs against...

... a few easy and fast things you can start doing right away to reduce your consumption in the lab: Turn...your oven on all day to save time making dinner at night, so why leave your lab equipment on? This applies...fact, some scientists speculate the -80 °C setting might have been a marketing campaign! MIT Green Labs has...

...accelerate research and discovery by improving access to high-quality scientific materials and information. Our... repository of valuable research materials that might otherwise be lost amid lab closures or project terminations...Providing essential services: We continue to offer high-quality DNA sequencing, viral vector preparations...

...only two options, but very little data on which might work in your application. It can be quite difficult...materials and methods section. This section will vary slightly depending on the application — flow cytometry,...Antibodies 101: Validation Antibodies 101: Selecting the Right Antibody...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....recognizes a simple dinucleotide PAM (N4CC), lending to high target site density (average every 8 bp). Nme2Cas9...

...look and feel of addgene.org. Some you’ll notice right away, while others may be less obvious — but every...you can use the links under the search box to go straight to your materials of interest!   Figure 1: The...bar New menu Most common activities can be done straight from the new search bar, but the menu just above...

... also selected for other key properties, notably high solubility, limited affinity for other proteins,...interactions, particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will...Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian...

... (top right corner of this post) is an example of a seemingly clean DNA sequence (no Ns in sight). If ...clean reliable DNA sequence Anything less and you might suspect contamination in your sample or consider...

...physicists, chemists, and mathematicians. We hope to highlight every science field as we expand and encourage... This would help you direct your content to the right audience. Avoid using scientific jargons. Someone...

...pleasure to announce that we once again reached new heights on the Addgene blog: we surpassed 60,000 views ...researcher at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia ossificans progressiva...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....identified 67 genera of Proteobacteria that this toolkit might be used in. Read the paper in ACS Synthetic Biology...