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  1. Cloning

    Type
    Guide
    ...Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO Gibson ... its multiple cloning site . Due to their short length, it is also easy to add restriction sites to any...custom ordered DNA oligos of 60-80 base pairs in length, with 30-40 base pairs of homology to the ends ...
  2. Retrovirus Guide

    Type
    Guide
    ...Questions (FAQ) about γ-Retroviral Plasmids How do engineered γ-retroviruses differ from lentiviruses? There...There are a few important differences between engineered γ-retroviruses and lentiviruses. First, these viruses...
  3. Plan Your Experiment

    Type
    Guide
    ...gRNAs that have been used successfully in genome engineering experiments. Using validated gRNAs can save your...Publications Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular...
  4. CRISPR Guide Update

    Type
    Blog Post
    ...the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements and enhanced...
  5. The 12 Days of CRISPR

    Type
    Blog Post
    ...the 1st day of CRISPR, Addgene gave to me: An engineered prime editor with enhanced editing efficiency...
  6. Lentiviral Guide

    Type
    Guide
    ...use the HIV protein tat in order to produce full length virus from the transfer vector during the viral...This deletion abolishes transcription of the full-length virus after it has incorporated into a host cell...
  7. Summer Fun at Addgene!

    Type
    Blog Post
    ...webinar “Navigating an Expanding Landscape for Engineered Systemic AAVs” with the director of CalTech’s...
  8. Our New Antibody Mascot is....

    Type
    Blog Post
    ...  This as-yet–unnamed friend is a huge fan of scavenger hunts. In fact, they’ve been following a set of...
  9. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls Feng New viral service, multiple serotypes Looking...
  10. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...dendritic spines!  Curious? Watch our AAV Data Hub Challenge recording to see two reports presented by the ...
  11. Editor's Choice, October 2016

    Type
    Blog Post
    ...Grad School Advice Part 2: Building Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare...
Showing: 601 - 620 of 802 results