...collaborates with NIH-funded researchers and utilizes grants to support our own initiatives, Addgene is deeply...developments. These abrupt changes threaten to slow transformative biological discoveries and life-saving medical...archiving their biological materials during funding transitions. Enabling resource sharing: Our operating model...services can be listed as direct costs from a CRO in grant applications, addressing the new funding structures...

...and packaging plasmids as well as transfer plasmids. Download the orange lentivirus background.         ...our fluorescent protein background. Download the orange fluorescent protein background.           Did...px or 1080 px).         Do you do a lot of transformations with the plasmids you receive from Addgene?...

...PGC-1a1, a transcriptional coactivator induced in skeletal muscle by exercise and endurance, protects ...have reported a mechanism based on studies of transgenic mice that helps to explain how it works. The ... skeletal muscle expression of kynurenine aminotransferases, to enhance the conversion of kynurenine into...

...weight by SDS-PAGE, and are subsequently transferred to a membrane. A primary antibody recognizing the protein...is added, and will bind to that protein on the membrane. Then, addition of a secondary antibody allows...showing GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common ...bind to proteins on the cell surface, or the cell membrane can be permeabilized to allow antibodies to bind...

.... Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection by Alyssa Cecchetelli...editing. Using a fusion between Cas9 and reverse transcriptase and a prime editing guide RNA, it can introduce...

...fluorescent tags in neurons. Their toolkit named ORANGE (Open Resource for the Application of Neuronal ...in neurons.  Read the paper in bioRxiv Find the ORANGE toolkit   Cas9/gRNA ribonucleoprotein delivery...delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages...for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the tetraloop of the...

...at Addgene, and send random sets of 4 people out to lunch.   I think these random lunches would be a wonderful...-make-time-for-that” type projects. Cross-team “Random Lunches” With more employees, Addgene has naturally...Kamens, responded by starting something we call “Random Lunches.” Once or twice a year, she’ll mix up the...

...). G. stearothermophilus grows at temperatures ranging from 30-74 °C, with an optimal growth temperature...or G) Spacer Length 20 nt 22 nt Temperature Range for Optimal Activity 35 - 45 °C 50 - 70 °C Genome...were incubated in reaction buffer at temperatures ranging from 25-80 °C. Activity is expressed as Kcleave...Harrington et al tested GeoCas9’s editing skills by transfecting HEK293 cells with GeoCas9 or SpCas9 RNP’s and...

...B) Strategy for leak-free transcriptional control. C) Iterative transduction workflow overview. Image ... by integrating a SL-insensitive fluorophore (LSSmOrange) as an internal control and a SL-sensitive fluorophore...fluorescent biosensor has a high sensitivity (50 nM-500nM range) and a high specificity for SLs in vitro, and they...

...sequences to sequences in a database ExPASy translation tool: Translates a DNA sequence using all six open reading...Annotates features, BLASTs, aligns, digests, and translates a sequence.   NEBCutter2: For finding restriction...information, such as expression patterns, post-translational modifications and polymorphisms Codon optimization...

... tools for diverse applications. For example, RANbodies can be used as an alternative to conventional ...sdAbs were used to create GFP scaffolds for transcriptional activation.  sdAbs in the clinic sdAbs have...also been some interesting work done developing transgenic mice that express camelid variable domains (aka...Llamas Required - Synthetic Nanobodies Against Membrane Proteins More resources from addgene.org  Plasmids...

...scientists and technology transfer offices trust us to work for them with full transparency. We don’t have to ...resources continuously raising money from donations or grants to fulfill their mission. Addgene is fortunate ...Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey, Randall, Ray, Rebecca, Renee, Shannon, Susanna, Tenisha...

... sensor AAV GABA is the primary inhibitory neurotransmitter in vertebrates, but methods for directly imaging...GABA sensor (pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant... through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used in conjunction ...pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]) of this membrane-embedding voltage detector. Find Archon1 and its...

...expression, researchers often need to determine these transduction patterns empirically for their own experiments...calcium dynamics that govern such activities as neurotransmitter release is vital in studying neural networks...GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience...research relies on accurate monitoring of neurotransmitters and we are excited to offer AAV for the newest...

...ahead of time included contacting YNP staff to arrange permits to visit places not accessible to the public...night was fun but also very tiring. It feels a bit strange to be using a micropipette and gloves at a campsite...similar to a type II-C Cas9 from Parvibaculum lavamentivorans, whose optimal growth temperature is 30°C. ...rRNA in bacterial RNA-Seq. This enriches for transcripts of interest without an increase in the number...

...in the cell. gDNA guides will also need to be transfected into the cells of interest. In contrast, RNA ...must be expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary structure can ...removal RNA-guided CRISPR enzymes cause double-stranded breaks, with either blunt (Cas9) or staggered ...Cpf1) cleavage. NgAgo takes a different approach, randomly removing 1-20 nucleotides from the cleavage site...

...using multidisciplinary, collaborative, and translational approaches for scientific tool and technique...However, careers outside of the lab highlight “transferable” skills such as leadership, management, and ...to publication and acquisition of funding. If a grant committee is not convinced that your project is ...Leadership Networking University technology transfer office positions   Market analyses Patent...

...This method involves measuring the absorbance/transmission of light through a liquid to determine the concentration...spectrophotometer measures these absorbances using UV-transparent cuvettes. First you measure the absorbance of...PicoGreen or SYBRGreen, are specific for double strand DNA (as compared to spectrophotometric methods ...However, the measurements are made in the visible range and can be read with a standard ELISA reader if ...

...ubiquitin ligase, the Kanemaki lab was able to translate AID to yeast, as well as mammalian and human cell...PubMed PMID: 31026591. Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed...are also thought to play a key role in the transcriptional regulation of many genes. A number of groups...thus far for these latter experiments, such as transcription factors tethered to dCas9, require long time...

...possibility, safety, and efficacy of the mtDNA transfer options being investigated; A UK regulatory body...emerging IVF techniques designed to prevent the transmission of mitochondrial disease;” The Nuffield Council...upon a moratorium until another meeting, three branches – a private funder, government regulators, and...question we are left with is not “what is the translational pathway for CRISPR/Cas9 research” but whether...