We narrowed to 961 results for: OMP;

...cassettes for selection of transgenic plants. The complete GreenGate toolkit, now available at Addgene, also...GreenGate can do is “save you half a year,” in comparison to more tedious and time-consuming traditional...traditional cloning methods, Lohmann said. The key component of Golden Gate and GreenGate are type IIS restriction...

... For more details, check out this comprehensive review comparing optogenetic CRISPR techniques (Matony...repeatability. The system developed by the Sata lab is composed of two fusion proteins: 1) the genomic anchor ...of transferrin receptor CD71 in HeLa cells.   Comparison of photoactivatable CRISPR strategies Publication...

...labels are obtained by independent audit, and companies need to pay to obtain them. There’s a database...you don’t see one, be an ACTivist and encourage companies to obtain these labels! Reusing plastic Historically...benchtop, high throughput washing devices that are compatible with a variety of pipette tips. This is part ...

...bird feces, which get eaten by another snail, completing its lifecycle. Halloween genes The Halloween ...grasshoppers expressed different proteins in their brains compared to uninfected grasshoppers, including those linked...Additional resources on the Addgene blog Read about company culture at Addgene See some of our previous Halloween...

...could take all of the credit, the success of the company is attributed to the entire Addgene family; the... on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as well as plenty of delicious...course of scientific research. What was once a complicated process of requesting and sharing plasmids has...

... come in the new year. I am proud of what we accomplished in 2023 and thrilled to be sharing our plans...addition, we released the MORF collection, a comprehensive transcription factor library, in lentiviral ... efficiencies typically adopted by for-profit companies, while making use of our nonprofit status to achieve...

... Blue light renders the NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress.... In the dark, the NLS is inaccessible and the complex remains in the cytoplasm. Read the paper in Cellular...CRISPR-based DNA demethylation Figure 2: The components of Casilio-ME1. Image from Taghbalout et al.,...

...dire:  The current system is unsustainable, hypercompetition is corroding values, we are shifting away ...experience and encouragement.  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs...such as the National Postdoc Association Core Competencies and the Science Careers’ myIDP). Dr. Galit Lahav...

..., yellow, cyan, blue, and orange spectra. A comprehensive list of cell markers all tagged with mEmerald...color variants. The mEmerald plasmids offer a comprehensive list of readily available targets since the ...work and the stage IV lung cancer that has now prompted him to deposit the tools he and his team created...

... expert (SME) at eScience Labs, an educational company that creates hands-on science lab kits and digital...with faculty members to make sure the courses encompass what they want to teach.” Besides working with...lab: write!” Caitlin did this by editing 15+ comprehensive exam proposals, a few PhD theses, and occasionally...

...information.” Addgene is here to help scientists accomplish their goals. Many nonprofits must spend a lot...level of positive revenue to build reserves so the company is sustainable. The difference is what nonprofits...-oriented values. Addgenies like the nonprofit company culture as well. We work every day to do a better...

...through biomedical research. The Camelid family is composed of camels, llamas, and alpacas (and their undomesticated...Arbabi-Ghahroudi, 2017)!      Figure 1: Comparison of a heavy-chain only antibody and single-domain...blog over the years and have described how they compare to other antibodies and affinity reagents. But ...

...change in pathology. If the disease pathology is complex, a model may be most effective. Once drug candidates...and generate physiologically relevant models. In complex diseases, it can aid in generation of multiple ...specificity of that target and validate the hit compound. The standard is to test the drug on models which...

...of this effort, members of the Rawlins Lab have compiled a new toolkit for performing genetics studies ...workflow combines thoughtfully designed plasmid components with Fluorescence Activated Cell Sorting (FACS...in high throughput screening for agrochemical compounds that have the potential to regulate the parasitization...

...transfer in vertebrates. The system is comprised of 2 components: 1) an SB transposase, the enzyme required...efficiencies of stable gene transfer using SB100x were comparable to stable transduction efficiencies of integrating...

...CRISPR delivery systems, modifications to the RNA components, and challenging target genomes, such as the ...foremost, from the custom software used to design complex genetic circuitry to the downstream output. Tuesday...digital and analog versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also...

...labs today are semi-synthetic or fully synthetic compounds. Antibiotics can be categorized based on whether...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...make sure your plasmid and E. coli strain are compatible! Check out this list of common E. coli genotypes...

...autoclave. Pro-Tip Once your autoclave cycle is complete, we recommend opening the door to the autoclave...

...proteins >150 kDa. Select Start Run. When the run is complete, select Done. Section 5: Blocking Prepare 1X TBST...

...used live virus (Cele et al., 2021). “We weren't completely shocked [with these results] because we were ... variant, which has only 9 different mutations compared to the wild-type, escaped neutralization. “That...SARS-CoV-2 variants, this study was the first to compare so many different strains. It was also the first...