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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 610 results for: GAN

Showing: 581 - 600 of 610 results

  1. Evolution of Lab Techniques

    Type
    Blog Post
    ... abundance. If a scientist wants to know if an organism has a particular gene mutation, southern blotting...

  13. Molecular Biology Reference

    Type
    Guide
    ...transcriptional machinery from a particular organism or group of organisms. Meaning, if a plasmid in intended ...abbreviated to A, T, C, and G respectively) that are organized into a double stranded helix. The order of these...the instructions to make every protein within an organism. Proteins are made up of amino acids. Each amino...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and even cultured...when trying to sequence an entire plasmid or an organism’s genome. One Sanger sequencing reaction would ...

  17. Plan Your Experiment

    Type
    Guide
    ...these principles apply to using CRISPR in other organisms. First, consider the genetic manipulation that...carry out your experiment in your particular model organism. There may not be a perfect plasmid for your specific...disrupt gene function in a particular cell type or organism without a specific preferred mutation Cas9 (or...sequence and design your gRNA. Know your cell line/organism and genomic sequence When possible, you should...may vary depending on the specific cell line or organism, which may affect the observed efficiency of CRISPR...

  20. CRISPR Guide

    Type
    Guide
    ...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG...Hultquist, J. F., Waters, C. S., McGregor, M. J., Krogan, N. J., & Bondy-Denomy, J. (2017). Inhibition of..., E. P. R., Lin, S., Kiani, S., Guzman, C. D., Wiegand, D. J., Ter-Ovanesyan, D., Braff, J. L., Davidsohn...23907171 Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss,...
Showing: 581 - 600 of 610 results