We narrowed to 614 results for: GAN

1. How to Negotiate a Successful Lab Start Up Budget

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   Blog Post

   ...provide value to others This is a non-profit organization. Therefore, collaboration and community is the...

2. Plasmids 101: Common Lab E. coli Strains

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   Blog Post

   ...blog post covering E. coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin...

3. Exploring Applications of the Bioluminescent HiBiT Tag

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   Blog Post

   ...Researchers can design drugs to bind to well-defined ligand-binding pockets or unique surface epitopes for ...

4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   Blog Post

   ...which allow AAVs to broadly spread throughout an organism, including difficult-to-target tissues like the...

5. CRISPR 101: Epigenetics and Editing the Epigenome

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   Blog Post

   ...a number of tools for this purpose. Epigenetics began as a correlative field in which covalent modifications...

6. Viral Vectors 101: AAV Variables That Matter

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   Blog Post

   ...j.neures.2014.09.002 Zingg, B., Chou, X. lin, Zhang, Z. gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, L. I...

7. Is this the right place for me? 8 tactics for choosing a lab

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   Blog Post

   ...encourage diversity in all forms? How are their organizational skills? Are they involved with colleagues ...

8. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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   Blog Post

   ...made targeted insertions relatively simple in C. elegans and bacteria. But CRISPRing typical mouse models...

9. Generating Mouse Models Using CRISPR/Cas9

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   Blog Post

   ...0471142905.hg1508s88 Leidy-Davis T, Cheng K, Goodwin LO, Morgan JL, Juan WC, Roca X, Ong ST, Bergstrom DE (2018...

10. Plasmids 101: Using Transposons in the Lab

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    Blog Post

    ... transposons are delivered to cells or a model organism, they insert into the host’s genome. Then, the...

11. A Guide to Getting Started in Undergrad Research

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    Blog Post

    ...at UC Berkeley. Most of the scientists I’ve met began doing research in their undergraduate years. We ...

12. Important Considerations When Using AAVs

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    Blog Post

    ...Anterograde axonal transport of AAV2-GDNF in rat basal ganglia." Molecular Therapy 19.5 (2011): 922-927. PubMed...

13. Sequencing Options for CRISPR Genotyping

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    Blog Post

    ...impact of CRISPR editing in the model cell line or organism. Several options exist for unbiased off-target...

14. Antibodies 101: Designing Your First Flow Panel

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    Blog Post

    ...different in nature (eGFP being a protein and FITC an organic molecule), their bleed is so strong that these ...

15. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post

    ...unaffected by intervening proteins, lipid bilayers, organelles or other cellular impediments. FRET has been ...

16. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post

    ...Cellular and Molecular Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the...

17. CRISPR 101: Cytosine Transversion Editors

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    Blog Post

    ... they showed that UNG from both E. coli and C. elegans could be effective components of CGBEs. Their eOPTI-CGBE...

18. Antibody Validation for Flow Cytometry

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    Blog Post

    ...efforts and information provided by external organizations like the Human Cell Differentiation Molecules...

19. Delivery Methods for Generating iPSCs

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    Blog Post

    ...embryoid bodies which are compact balls of loosely organized tissues that express markers of all three germ...

20. Chemogenetics Guide

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    Guide

    ...small molecule ligand. The ideal chemogenetic tools are unresponsive to native ligands and are engineered...transmembrane proteins that bind a specific ligand. Ligand binding then activates G-proteins to modulate...unresponsive to endogenous ligands, could be activated by synthetic ligands. Specifically, Receptors Activated...insensitivity to endogenous ligands, have low constitutive activity, and their activating ligands have few, if any... Figure 2: DREADDs, their ligands, and signaling properties. DREADD Ligands Muscarinic-receptor based ...neurotransmission. This, and the fact that GPCR ligands can have high potency, which is potentially important... Activated Solely by Synthetic Ligands (RASSLs) based on κ-opiod receptors (Coward et al. 1998). These...