...Reagents (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used to...and nonprofits only. You may also like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot Wolfe... ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nature Protocols . 2009;4(10):1471-501. doi:...requires OPEN pools (available by request from the Joung lab) and the following reagents (listed below and...1997 ) and later modified by Pabo and colleagues ( Joung et al., PNAS 2000 ). The Oligomerized Pool ENgineering...protocol for practicing OPEN is available in the 2009 Joung Nature Protocols paper . How to Cite this Kit These... used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section...

...This guest post was contributed by Johnny Kung, Director of New Initiatives for the Personal Genetics...public on the implications of their work. Johnny Kung is the Director of New Initiatives for the Personal...

...insert FLAG tags into the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult...microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID: 26701308. Additional...

...modified version of the Python program described by Joung et al. 2017, which we hope to make available as ...:664–678 . https://doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt...

...GeCKO v2) 123,411 20 bp 600–1200 ng Solid Joung et al. Nat Protoc. 2017 CRISPR Knockout Human..., plus a 24 bp barcode 250–1,000 ng Solid Joung et al. Nat Protoc. 2017 with modifications Screening...

...Mobile-CRISPRi plasmids at Addgene! References Auchtung, Jennifer M., et al. "Biology of ICEBs1, an integrative... 86 (2016): 14-25. PubMed PMID: 27381852. Choi, Kyoung-Hee, et al. "A Tn7-based broad-range bacterial ...

...retired! Conversely, cells younger than the 3rd – 4th passage are too young and should not be used for...

...model organisms, including bacteria, alternative fungi (C. albicans, pC2HB) (7) and mammalian cells. If...codon adapted two-hybrid system for the pathogenic fungus Candida albicans. Nucleic Acids Res. 2010;38(19...

... as anthocyanin production, or disease, such as fungal growth. Healthy plants produce lots of seeds. More...mean more chances to recover a transformant. Use young plants: If you transform early, your plants will...

...specificity. 2018. Lee JK, Jeong E, Lee J, Jung M, Shin E, Kim YH, Lee K, Jung I, Kim D, Kim S, Kim JS. Nat Commun...in genome imaging.. 2018. Ma H, Tu LC, Naseri A, Chung YC, Grunwald D, Zhang S, Pederson T.. Nat Methods...Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. Nature . 550(7676):407-... Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA,..., Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F. Science . 356...Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F. Science . 358(6366):1019-1027. PMID:...Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev...

...overcome this problem in C. roseus by working with young seedlings and optimizing several other aspects of...re-invent the wheel. Test different leaf ages Usually younger leaves work better. Not every leaf is equally susceptible...

...Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing with CRISPR-Cas13....Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim J-S (2017) In vivo...

...within cell-free genomic DNA, and detect COVID-19 (Joung, Ladha et al., 2020). Cas13a in mammalian cells ... 26593719. PubMed Central PMCID: PMC4660269. 7. Joung J, Ladha A, et al., ‘Point-of-care testing for COVID...

...14704360. PubMed Central PMCID: PMC373311. 3. Kim, Jung-Sik, et al. "Epitope tagging of endogenous genes...18784188. PubMed Central PMCID: PMC2577350. 4. Kim, Jung-Sik, et al. "Intact cohesion, anaphase, and chromosome...

...the procedures in the lab are done with robots. Young scientists going into this field don’t really get...position in academia. It is therefore important for young scientists and PhD students to also explore a range...

...Corsino. When someone says “internationalization” young hearts often shake with the fear of uncertainty....entrepreneurial visions thereby opening new frontiers for young talent around the world. The wealth of knowledge...

..., J. D. Sander, J. Staber, S. J. Wheelan, J. K. Joung, P. B. McCray, F. D. Bushman, P. L. Sinn, and N....Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs.,”...

... a nucleotide triplet and when several ZFNs are strung together, it can read many base pairs simultaneously...Pickle CS, Ralston EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov ...

...this represents a major downside in optogenetics (Belzung et al., 2014). This could be overcome by fusing...28.28 (2008): 7025-7030. PubMed PMID: 18614669. Belzung, Catherine, Marc Turiault, and Guy Griebel. "Optogenetics...

...TALengineering Reagents Joung Lab TAL Effector Engineering Reagents You may also like... Keith Joung Lab plasmids...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and...plasmids Dan Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed three platforms for engineering...pages describing these reagents are provided below: Joung Lab REAL Assembly TALEN Kit Individual REAL TALE...