...constitutively expressed to identify successfully transfected cells. C-to-T editing by the APOBEC-Cas9 editosome... to cells segregated solely by a reporter of transfection, which only report the efficiency of plasmid...frequency of base edits of interest than solely transfected cells. “Gene On” (GO)- a functional reporter ...

...(see paper) Transfection none, need Cas9 plasmid Calarco 47943 pMB70 cU6 BsaI Transfection none, need ...

...constitutive promoter and can be used to monitor transfection or viral infection efficiency, to study changes... Renilla luciferase is used to monitor viral transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623... Firefly luciferase is used to moniter viral transfection Sidi Chen 109396 TBG-Vector Firefly TBG AAV-... Firefly luciferase is used to moniter viral transfection Sidi Chen 118412 ssAAV-EF1a-FLuc-WPRE-HgHpA_...

...interference (RNAi). The most common method is transfection of short interfering RNA (siRNA). Knockdown .... This approach often involves the transient transfection of expression plasmids in a given cell line....antibody should be able to detect even in cells transfected with low efficacy. But since there can be large...differences between endogenous expression and transfected overexpression, this approach cannot confirm ...

... number of plasmids that are required in the transfection step of the viral packaging cell line. For example...resources on addgene.org Addgene Protocols: General Transfection The Addgene Retrovirus Guide  ...supplied by three types of plasmids, which are cotransfected into the 293T packaging cell line. This system...

...expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light...contain low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified... The fluorescence pattern is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections...antibody. Protein expression is compared to an untransfected control and the protein size is confirmed. Residual...

...base pairs on each side, in fact. Later, when we transfect the cells, these large regions of homology enable...being cut and destroyed by CRISPR/Cas9 during transfection. Making the double strand break and introducing...

... your packaging cell line, prepped your DNA, transfected and harvested your lentivirus. Now it’s time ...the correct transfer plasmid was used in the transfection; if preparing several different lentiviruses...

...ratio of acceptor to donor fusions varies with transfection efficiency and any unpaired fluorescent proteins...measure (overexpressing a gene or protein after transfection). Future of FRET The first genetically encoded...

... proteins and the antibodies are produced by transfecting mammalian cell lines and collecting the secreted...

...interfering RNA) via transfection, or shRNA (short hairpin RNA) via transfection or viral delivery of ...

...the University of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a ...primers targeting the transfer plasmid used in the transfection. PCR products are analyzed by gel electrophoresis...

...in mammalian cells is relatively quick. After transfection, HEK-293T cells start producing virus within...systems, it is standard to do only one passage (transfection). This differs from typical insect cell preparations...

...be produced through a single or dual-plasmid transfection, depending on whether the heavy and light chain...they’ve been separated onto two. You’ll first transfect your cells with your plasmid(s), and then wait...

...BiFC constructs are expressed transiently by transfection or stably impacts the success of BiFC experiments...dose-dependent manner. This is straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly...

...packaging and host genome integration. After co-transfection of these plasmids, 293T cells released transgene-containing...work with primary cells or other difficult-to-transfect cells. Lentiviral barcoding libraries have been...

...identical targeting vectors delivered through transfection of naked DNA. Other investigators who had been...then packaged into AAV virions via transient transfection of 293T cells with helpers, and used to infect...

...generating hiPSCs for clinical purposes: mRNA transfection- this allows transient expression of the reprogramming...express reprogramming factors To date, the mRNA transfection method has not been used successfully to derive...

...site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA sequences...efficacy of your gRNAs is then a simple matter of transfecting HEK293T cells with your modified pCAG-EGXXFP...

...the passage number of a culture the lower the transfection and transduction efficiencies. Cultures should... while others opt for a single harvest after transfecting with the transfer and packaging plasmids. A ...