... Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with 10 guides...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic...than NHEJ. Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled...CRISPR expression plasmids that have been designed for use in parasites. CRISPR... CRISPR Parasites CRISPR Plasmids...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...

...information on these new CRISPR screening tools, see our CRISPR/Cas Plasmids: Pooled Libraries webpage or read ... These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering a lentivirus-...Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening. Also, check out our updated CRISPR-Cas ...CRISPR-Cas resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational videos, download...Tet-inducible CRISPR activators and repressor plasmids. (Kearns et al., Development. 2014..) CRISPRs for Xenopus... Hot Plasmid list. Other popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies...Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems have been...

... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled Libraries Jump To...make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells. DIVERGE: Positive... In cDNA libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains...Overview of what pooled libraries are; how to amplify and use a pooled library; and what types of screens...Introduction to Pooled Libraries Pooled libraries represent a powerful tool for forward genetic screening, or identifying...simplest level, pooled libraries are single preparations of many different plasmids. Plasmids within a given... name, pooled libraries are supplied as a mixed population of plasmids in a single tube. Pooled libraries...

...used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout, activation...preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used ...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...you need to use CRISPR in your own lab. Check out our CRISPR resources or browse plasmids below by function...Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and the basics...planning your first CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've...analysis, discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...

...31. CRISPR-Display: A novel CRISPR technology for targeting RNA cargos to specific DNA loci John...Addgene Blog Read Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts ...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ... control over CRISPR/Cas9 mediated genome modification and gene expression. The plasmids associated with...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase...whereas the nonsequestered pool goes on to initiate translation. These TRICK plasmids provide valuable tools...

...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...gene, so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually...For more detail on using CRISPR libraries for screens, see our pooled library guide and our blog post ...

...(2015) Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Nat Methods... function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences...using oligo library synthesis. Prior to Cas9 activators, similar tools were made using other DNA binding ...group has develop several popular CRISPR-based tools for altering DNA sequence or regulating gene expression..., Wang X (2015) WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system. Genome... More about Genome-wide Screens with CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on...Catch Up on Your CRISPR Background with Our CRISPR Guide Pages ...

...Cas12a plasmids Addgene's full CRISPR guide and summary of CRISPR pooled libraries . FlipGFP-based activity...useful for detection assays and more. Pooled Libraries : Pooled libraries of spike protein mutations. Research...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...SARS-CoV-2 plasmids are curated on a separate Ginkgo Bioworks Plasmid Collection page. SARS-CoV-2 Pooled Libraries...to ask them to deposit the plasmid with us. Return to top Pooled Libraries SARS-CoV-2 Spike (S) Ectodomain...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...

...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...precise DNA-targeting of CRISPR-Cas systems, allowing for the integration of large fragments of DNA at specific... or search the tables below for CRISPR Transposase plasmids. Plasmids are available for expression in ...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...

... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand DNA degradation.... or search the tables below for CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...

..... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts In collaboration with the labs who...deposited CRISPR plasmids , we've created a series of links and guides to help you use CRISPR in your lab... to identify potential DNA target sites for TALEs and CRISPR/Cas. CRISPRdirect From the Database Center...PDF 2.3 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9... curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community... CRISPR References and Information CRISPR References and Information You may also like...... More Guide to CRISPR technology Addgene Blog Posts How to Design Your gRNA for CRISPR Genome Editing ...

...Click here to view all pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections...ORFs Synthetic Biology Pooled Libraries Pooled libraries are a set of plasmids all built with the same...curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 156,627 plasmids . We've...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...

...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas... the Doudna Lab . Browse, sort, or search the tables below for CRISPR nickase plasmids. Plasmids are available... CRISPR Nick CRISPR Plasmids: Nick...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...Nick Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...

...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...gene, so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually...For more detail on using CRISPR libraries for screens, see our pooled library guide and our blog post ...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying...search the tables below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR... CRISPR Purify CRISPR: Purify Genomic ...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...

...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...by the Doudna Lab . Browse, sort, or search the tables below for CRISPR RNA targeting plasmids. Plasmids...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In bacteria...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert...

...CRISPR plasmids that create double strand breaks in DNA.... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully...maintained by the Doudna Lab . Browse, sort, or search the tables below for CRISPR plasmids designed to introduce...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...

... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...

... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ... The following CRISPR plasmids have been designed for use in Xenopus. Cut Fully functional Cas9 enzymes...in the DNA, possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...maintained by the Doudna Lab . Browse, sort, or search the tables below for CRISPR plasmids for RNA editing...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR... CRISPR RNA Editing CRISPR Plasmids...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing...