Even more elegant: Single injection CRISPR/Cas9 in C. elegans
Type
Blog Post
...time.
Creating a modular, selection-based gene tagging approach
Multiple features of C. elegans have made... between a fluorescent protein (FP) and a 3xFLAG tag (see construct schematic below.) After self-excision...intron, and only the fluorescent protein and 3xFLAG-tag sequences are inserted into the genome.
Self-excising...template plasmid to create fluorescent and 3xFLAG-tag fusion proteins.
The repair template and Cas9-sgRNA...Dickinson et al illustrating the steps to fluorescently tag a C. elegans protein. The his-72 locus is shown as...little hands-on labor and could facilitate the tagging of every protein in the C. elegans genome. Although... gene tagging...