...NETRF strongly encourages its funded scientists to make their published materials and resources available...

... by choosing the “CRISPR/Cas: Design oligos for making guide RNAs” link under the “Support Tools” heading...

...Protocols gRNA Design Tools CRISPR Blog Posts To make targeted epigenetic modifications, researchers have...

... require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. The T375G mutation...

... by choosing the “CRISPR/Cas: Design oligos for making guide RNAs” link under the “Support Tools” heading...

...sequences of oligonucleotides to be synthesized for making specific gRNA pairs. ZiFiT Targeter is freely available...

...shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2 ...

...purified DNA from the original plasmid that was used to make the virus. For more details, see our FAQ on What...

...define standard prefix and suffix sequences that make it easy to combine components for synthetic biology...

...102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies) against membrane...

...Engineering blog post to see how Addgene depositors are making this possible in a range of organisms. Find CRISPR...

...hermaphrodite consists of 959 somatic cells, which makes them attractive for imaging and fluorescence microscopy...

...nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart research model. With more...

...components Trono 63586 psPAX2-D64V 2nd Packaging For making Integrase deficient lentivirus Rawlings , Scharenberg...

... Transgenic Expression in organisms pBigT - For making transgenic mice. Contains a loxP-flanked...

...vector, CMV promoter, and Blasticidin; can be used to make cell lines Tet-On Advanced; plasmid 26730 has hygro...

...Symptoms include memory loss, deficits in decision-making and language abilities. The disease can be classified...

...and Yeast expression Spike protein constructs for making VSV pseudo virus Mammalian constructs Due to the...

...pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207...

...generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect... Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. ...very close proximity to the region to be edited, making the PAM sequence (NGG) limiting. This PAM is abundant...Anti-CRISPR Small Precision Edits CRISPR can be used to make small, very precise edits of a handful of bases,...RNA-binding protein to stabilize pegRNAs Twin PE - two PEs make complementary edits to opposite strands Prime editing...the ability to target almost any genomic locus, makes CRISPR an ideal genome engineering system for large-scale...large-scale, forward genetic screening. CRISPR can make highly specific, permanent genetic modifications...