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...enhancer Viviana Gradinaru 99133-AAV1 pAAV-CAG-fDIO-mNeonGreen An AAV genome with Flp recombinase-dependent ...Cre-dependent recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies... tools and emphasizes their rapid and broad dissemination to the research community. Browse plasmids ,...83894-AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV9 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...

...protein kinase kinase kinase 14 FTDCR1B, HS, HSNIK, NIK MAP3K8 mitogen-activated protein kinase kinase kinase...protein kinase kinase 1 MAPKK1, MEK1, MKK1, PRKMK1 MAP2K2 mitogen-activated protein kinase kinase 2 FLJ26075...Kits and Collections Human Kinase ORF Kit : 558 distinct human kinases and kinase-related protein Open Reading...expression. Myristoylated Kinase Kit : Consists of 190 human kinases and kinase-related open reading frames...phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase, catalytic, gamma...phosphoinositide-3-kinase, regulatory subunit 1 (alpha) GRB1, p85, p85-ALPHA PIK3R2 phosphoinositide-3-kinase, regulatory...plasminogen activator, urokinase ATF, UPA, URK, u-PA PLAUR plasminogen activator, urokinase receptor CD87, UPAR...

...dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing...2014. INTRSECT INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic molecular... Cre and Flp (B,E). When the correct combinations of recombinases are present, the introns are excised...expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral...Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral...intersectional cell population definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional...expressed in cells based on a doubly-specified combination of genetic and/or anatomical-defined parameters...

...Mitogen-activated protein kinase kinase MET MET proto-oncogene, receptor tyrosine kinase MLST8 MTOR associated...STK4 Serine/threonine kinase STK11 Serine/threonine kinase 11 TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2... CDK6 Cyclin-dependent kinase CNKSR CNKSR1 CNKSR2 Connector enhancer of kinase suppressor of Ras CYTH2...receptor tyrosine kinase 2 ERK MAPK1 MAPK3 Also known as MAPK; Mitogen-activated protein kinase ETS ETS1 ETS2...coiled-coil containing protein kinase ROS1 ROS proto-oncogene 1, receptor tyrosine kinase RPS6KA RPS6KA1 RPS6KA2... Ribosomal protein S6 kinase RPS6KB RPS6KB1 RPS6KB2 Ribosomal protein S6 kinase B1 RPTOR Regulatory associated...within the cell. GTPases have an active (GTP) and inactive (GDP) state, regulated by the addition and removal...

...Flpo recombinase Recombinases Li Zhang 121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent Cre recombinase Recombinases...expression Recombinases James M. Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases James...expression Recombinases Rylan Larsen 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases James M....expression Recombinases Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Karl Deisseroth...expression Recombinases Karl Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases Karl Deisseroth...expression Recombinases Hongkui Zeng 51669 AAV phSyn1(S)-FlpO-bGHpA Syn Flpo expression Recombinases Hongkui...expression Recombinases Ian Wickersham 183412 pAAV-CAG-FlpO CAG Flpo expression Recombinases Janelia Viral...

... catalyze cytidine deamination instead of (or in addition to) adenosine deamination, producing cytosine...Plasmids: CRISPR transposases CRISPR Recombinases and Integrases Recombinases, and their subfamily integrases...interesting adaptive immune response that detects and eliminates foreign DNA, which scientists have modified for...Overview of the basic CRISPR mechanism. CRISPR was originally employed to knock out target genes in various...SpCas9, has one active nuclease domain and one inactivated one, allowing it to generate DNA nicks by cutting...cutting only one strand. Catalytically inactive “dead” Cas9 (dCas9) contains mutations in both nuclease...backbone HypaCas9 — increase Cas9 proofreading and discrimination evoCas9 — decrease off-target effects xCas9...

...displays enhanced transduction of the retina following subretinal administration and enhanced transduction...transduction and lateral spread in the retina following subretinal injection. It was identified by John ...transduction and lateral spread in the retina following subretinal injection as compared to the parental...Heparan Sulfate Binding on Transduction of Retina by Recombinant Adeno-Associated Virus Vectors. J Virol ...may also like: Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part ...possible) in vivo testing, and full sequencing of the final viral vector preparation. These viral vector preparations...delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes were ...

...Cre Recombinase James M. Wilson AV-1-PV2676 105553-AAV1 pENN.AAV.hSyn.Cre.WPRE.hGH Cre Recombinase James... Cre Recombinase James M. Wilson AV-2-PV1090 105537-AAV2 pENN.AAV.CMVs.Pl.Cre.rBG Cre Recombinase James...Cre.SV40 Cre Recombinase James M. Wilson AV-5-PV2408 105550-AAV5 pAAV.GFAP.Cre.WPRE.hGH Cre Recombinase James... Cre Recombinase James M. Wilson AV-8-PV1090 105537-AAV8 pENN.AAV.CMVs.Pl.Cre.rBG Cre Recombinase James... Cre Recombinase James M. Wilson AV-9-PV1090 105537-AAV9 pENN.AAV.CMVs.Pl.Cre.rBG Cre Recombinase James...pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 Cre Recombinase James M. Wilson AV-9-PV2394 107788-AAV9 AAV.rTH.PI.Cre.SV40 Cre Recombinase James M...Cre Recombinase James M. Wilson AV-9-PV2676 105553-AAV9 pENN.AAV.hSyn.Cre.WPRE.hGH Cre Recombinase James...

...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for ... to NeuN, plus Cre recombinase 60228 : sgRNA targeted to LacZ, plus Cre recombinase 60229 : sgRNA cloning... backbone with Cre recombinase 60230 : sgRNA targeted to NeuN, plus Cre recombinase-2A-EGFP-KASH 60231...plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes as well as non-coding...consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2...targeting efficiency. These plasmids use the inverted terminal repeats (ITR) from AAV serotype 2. SaCas9 only...KRAS , p53 , and LKB1 , plus luciferase-2A-Cre recombinase, and Kras G12D HDR template 60225 : control for...

...pCAG-T7-TALEN(Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed...TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0...new destination vectors can be used to create TALE-transcription factors and are used at the final assembly...pC-GoldyTALEN and RCIsricpt-GoldyTALEN are designed as destination vectors for the Voytas lab Golden Gate TALEN ...-BB and pTAL6-BB are designed as alternative destination vectors for the Golden Gate TALEN kit (Bogdanove...expression vector resulting in shorter N- and C-terminal tal protein segments (136AA and 63AA, respectfully...several later studies. pCAG-T7-TALEN(Sangamo)-Destination vectors are available with homodimeric or enhanced...

...sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly fusions in Drosopholia...Gaussia, Cypridina EF1α, CMV Dual secreted luciferase reporter (EF1α-Gaussia luciferase, CMV-Cypridina luciferase...luciferases ( CBRluc and CBGluc , respectively), Cypridina luciferase , and Luciola luciferase . Each type...87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale... pLenti6.2-ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival... pLenti6.2-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral...87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale...

...the N-terminal portion of split mCherry, while a phosphobinding domain is fused to the C-terminal mCherry... of kinases of interest by mass spectrometry in a technique called serine-oriented library/kinase-library...overall improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli...NEDD4-2 (Pooled Libraries #111705-8) . Screening kinases The Mode #1 phosphosite library can also be synthesized...pSerOTS described in: Mohler, K., Moen, J. M., Rogulina, S., & Rinehart, J. (2023). System‐wide optimization...: Barber, K. W., Muir, P., Szeligowski, R. V., Rogulina, S., Gerstein, M., Sampson, J. R., Isaacs, F. ...Lou, H. J., Turk, B. E., & Rinehart, J. (2018). Kinase substrate profiling using a proteome-wide Serine-Oriented...

...gene therapy development. In order to produce recombinant AAV vectors, researchers need to use three plasmids...the AAV vector genome), flanked by two inverted terminal repeats (ITRs). This table contains a general ...AAV packaging plasmid, expressing Rep2 and Cap2 Melina Fan , Addgene Research Program 232922 pAAV2/5 AAV5...AAV packaging plasmid, expressing Rep2 and Cap5 Melina Fan , Addgene Research Program 240485 pAAV2/6 v1...controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S PHP.S AAV packaging ...controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1 PHP.V1 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10 B10 AAV packaging...

...pAAV-mDlx-NLS-mRuby2 Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG...light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase iDreV Zeng ... Engineered systemic constructs from Viviana Gradinaru's lab at Caltech. Viral...engineered systemic serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link...possible) in vivo testing, and full sequencing of the final viral vector preparation. Systemic Delivery AAV ...NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER Center (Link opens ...CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV....

...Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...Innovation (IPI) Collection! Here, you’ll find unique recombinant antibodies targeting axonal guidance receptors...window) and an optimized sorting process. This combination allows IPI to generate antibodies to highly conserved... Axon Guidance Antibody Collection comprises recombinant monoclonal antibodies with high specificity for...accessible toolset for scientists worldwide. ID Recombinant Antibody Description Isotype Reactivity Recommended...Netrin, DCC, and IGDCC3 (PUNC) Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Applications PI Browse ROBO and Slit Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...

.... Pairs from methanogens like Methanosarcina barkeri and Methanosarcina mazei are orthogonal both in bacteria...strain with UAG termination function removed (RF1 is deleted); ompT, rne, lon inactivated Michael Jewett...strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA inactivated Michael Jewett...strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated Michael Jewett...Mb-Fluoro-Phe B5 Pyl Fluoro-Phe B5 M. barkeri fluorinated phenylalanine derivatives Mammalian TAG Ryan ...Mb-Fluoro-Phe D6 Pyl Fluoro-Phe D6 M. barkeri fluorinated phenylalanine derivatives Mammalian TAG Ryan ...sites changes to UAG, RF1 function removed, ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church...

...absence of unexpected DNA contaminants. The data is also used to determine recombination rates in FLEX/DIO/fDIO...Endotoxin contamination in vector preparations can alter the immunogenic properties of the final product...mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event...is performed on viral genomes isolated from the final AAV preparation. Sequencing results are analyzed...particularly in large animal studies. Endotoxin contamination is minimized by using an endotoxin-free plasmid.... To minimize the immunogenic properties of the final vector preparation, the quantity of gram-negative...Takara, and is routinely tested for mycoplasma contamination using mycoplasma detection kits. The cell line...

...Verdoes Backbones for mouse recombinant antibodies A panel of recombinant monoclonal antibodies against... Collection Addgene distributes ready-to-use recombinant antibodies made from plasmids in our collection...alternative approach is to use synthetically produced recombinant antibodies, created by cloning antibody components...Some scientists choose to work with full length recombinant monoclonal antibodies (R-mAbs) expressed in mammalian...research applications from the NeuroMab/Trimmer Lab Recombinant mAb Collection . Once the plasmids are transiently...of production due to their small size. These recombinant antibody fragments can be used in experiments...tool kit for rapid cloning and expression of recombinant antibodies. Sci Rep. 2014 Jul 30;4:5885. Andrew...

...Antibodies produced this way are called recombinant antibodies. Recombinant antibodies are created by cloning... time and labor intensive. Recombinant expression validation A recombinant expression strategy is used...cultured immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals... of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced from plasmids... and can either be used while suspended in the original media or isolated and placed in a buffer. Antibody... binding site. This process, known as V(D)J recombination, allows for a wide variety of antibodies to ...abundant isotype in blood, also found in tissues; predominant isotype in secondary immune response Monomer ...

...growth and proliferation. mTOR, a serine/threonine kinase, is found in two complexes, mTORC1 and mTORC2... and multiple familial cancers are caused by inactivating mutations in negative regulators of mTORC1. ...homolog LKB1 Also known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin...S6K Also known as RPS6KB1; Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von ...LST8 homolog GSK3 GSK3A GSK3B Glycogen synthase kinase 3 MDM2 MDM2 proto-oncogene mSin1 Also known as ...as MAPKAP1; Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin ...; tumor protein p53 PDK1 Pyruvate dehydrogenase kinase, isozyme 1 PI3K Catalytic Subunits PIK3CA PIK3CB...