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We narrowed to 51 results for: golden gate

Showing: 41 - 51 of 51 results

  1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...Strains Plasmids 101: TOPO Cloning Plasmids 101: Golden Gate Cloning Read all of our Plasmid Cloning Posts...recombinant plasmid to propagate. Any cells that contained non-recombinant vectors (re-ligated empty vectors, ...ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate while expressing...recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as well as several others...focus on a unique gene that makes the popular GatewayTM method possible: ccdB. But what is ccdB, what ...expressed the ccdB gene and were therefore unable to propagate because CcdA wasn’t available to counteract the... to thoroughly screen colonies for the insert. Gateway® technology (developed by InvitrogenTM) is essentially...

  2. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...test different ideas. We are a huge fan of the Golden Gate based MoClo system. The vectors mentioned in ...expression method enables us to quickly screen and investigate transcription factor candidates and the regulation...

  3. MXS Chaining

    Type
    Blog Post
    ... Method Technology Pros Cons References Golden Gate Type II restriction enzymes Can assemble up to...

  4. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...Blog: Learn How to Verify Your Plasmid Try Out Golden Gate or SLIC Cloning Read Other Plasmids 101 Posts...as this will be useful for the ligation step. 3. Ligate Your Insert into Your Vector In the ligation step...

  6. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial micro RNA and short hairpin... of CRISPR technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi..., gene knockout strategies cannot be used to investigate essential genes. Historically, gene silencing... which allows easy creation of long hpRNA with Gateway-based cloning and straightforward positive selection...from Worcester Polytechnic Institute where he investigated how plants achieve polarized cell growth in ...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...engineering. These components are provided in Golden Gate compatible backbones that can be combined with...all the latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying the nervous system...system The Cole lab recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists...neurobiology and neuorodgenerative diseases. The Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit...empty vectors that have standard cloning sites or gateway compatible cloning sites for easy cloning of your...toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst the...2007. PubMed PMID: 17937395 Listen to Our Tol2 Gateway-Compatible Toolbox Podcast Segment The impact of...

  10. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...a microscopist, a biostatistician, and that one golden-handed technician who can do a certain difficult...someone to coordinate, someone to decide, someone to delegate, etc. If you find that there is not someone (or...

  11. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... John W. Campbell. This period is termed as the golden age of SciFi, but the common themes that these ...between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called pegRNA (prime editing...
Showing: 41 - 51 of 51 results