...protein (probably while it is being synthesized) can form a complex with an ssDNA guide. Therefore, researchers...fraction of nascent NgAgo protein molecules manages to form a functional complex with ssDNA leading to inefficient...NgAgo (or a novel endonuclease) protein that can form a complex with a 5’-P-ssDNA guide at room temperature...necessarily represent the views of Addgene. Addgene performs Sanger sequencing on select regions of all distributed...plasmids as part of quality control, but does not perform functional tests. The newest genome engineer sharing...researchers were having difficulty achieving indel formation (gene disruption) with this new technology. To...respondent out of 88 confirmed successful indel formation at a target site. Also, 41 respondents attempted...

...(hence the name), which is due to a chromophore formed from a maturation reaction of three specific amino...aspects of GFP was the fact that the chromophore forms spontaneously and without additional co-factors,...efficiency at 37C Y66W Causes the chromophore to form with an indole rather than phenol component (cyan...between two domains of a protein that undergoes conformational change. Typically, two fluorescent proteins...

...team of physicians and scientists.  Although the form of malignant mesothelioma that affects the lining...widely understood and often misdiagnosed. Like other forms of mesothelioma, there are currently no targeted...model to identify SMN1 interacting partners and perform whole animal screens for small molecules that block...this little-known disease. You can find more information about the disease on the Meothelioma Cancer Alliance...

...and ATPase domains. To function, the Rep proteins form oligomers of Rep 78/68 or Rep52/40. Rep78/68 play...capsid proteins VP1, VP2, and VP3, which together form the outer capsid shell that protects the viral genome...

...Sattarzadeh et al. found that only the protonated form of GFP is photoconverted, so this technique may ...been encountered by some investigators when using forms of mEos." The ability to repurpose GFP is great ...

...limitations in the use of peptide tags, which often form only weak and reversible bonds. Howarth’s team has...Acad Sci U S A. 2014 Mar 17. PubMed) Peptide tag forming a rapid covalent bond to a protein, through engineering...

... an autosomal recessive, severe, childhood-onset form of blindness name Leber’s Congenital Amaurosis (...years to obtain FDA approval and make history in the form of Luxturna™. A better vector for ocular gene therapy...non-dividing cells because they remain in episomal form in the cell’s nucleus. But since nothing’s ever...

...stored in a retrievable format, we too rarely preserve “raw” data of any sort in a form that can be parsed ...underlying data easily available, organized in a form that can be understood by others, balanced against...should recognize that storing data in a sharable form is indeed a significant effort. Our standards of...publications must contain enough information (or links to information) for assessment of veracity of the...unprocessed and in capture format. This raises another issue of proprietary data formats. Many instruments store... raise the alpha isoform of GSK-3 to the same (deserved) stature as the beta isoform. Follow him on Twitter...Not quite. The PLOS policy on data access is a formal statement that is already practiced or required...

...movement, and assembling the many pictures together to form the full movement. Producing stop motion videos ...quirky lab story you’d like to see portrayed in LEGO form, feel free to share with us at [email protected] Science” was born. We chose the stop motion format for our videos as it is the most approachable for...

...designed to pay attention and enjoy anecdotal forms of information — identifying with a character, building...you can leave out. Start viewing your work in the form of a story, and you’ll very quickly get better at...Here’s an example of storytelling to answer an informational interview question: Interviewer: “Tell me about...

...applications of genome engineering - such as certain forms of gene therapy - avoiding the use of viruses is...different fish species in a variety of non-functional forms and is believed to be millions of years old. The... is one such system which has been designed to perform gene transfer in vertebrates. The system is comprised...

...each request, the depositing organization signs one form that can be used for every MTA request going forward...to find out your plasmid hasn’t shipped because a form needs to be signed. But the truth is, meaningful...There are many different flavors of MTAs, but the Uniform Biological Material Transfer Agreement (UBMTA) ...articles, and our MTA YouTube video for more information. Happy transferring!    Many thanks to our Tech...

... The disadvantage is that the GST moiety, which forms dimers, may influence binding kinetics of the target...molecule so, theoretically, saturated avidin could form tetramers of biotinylated protein. However, oligomerization...proteins on avidin can be controlled easier than formation of GST dimers. Avi-Tag plasmid for improved biotinylation...joyful that getting to the final step and the transformed bacteria refuse to grow. Eventual success was...

...growing the cells on solid plates, where each transformant forms its own colony, uninfluenced by the growth..., your NGS provider should return results in the form of many, many short DNA sequences called reads. ...cells when performing amplifications, and we use electroporation to ensure a good transformation efficiency...Typically they’ll be in a file in FASTQ format, a standard format for storing NGS reads and their corresponding...of the uniformity of the library distribution, with an AUC of 0.5 representing perfect uniformity (see ... seems straightforward: E. coli bacteria are transformed with the library DNA, they grow and replicate... Each Addgene library has its own depositor transformation and amplification protocol, but the overall...

...something differently. Communication comes in many forms—from transparency about data to allow others to ...good scientific citizenship in all its different forms as part of graduation, hiring, funding, and promotion... again, the problem comes down to inadequate information about what the original researchers actually ...ourselves having to ask authors for additional information (accession numbers, reagent details, author contributions...published. To a scientist, all of this checking and formatting can feel like yet another hurdle in front of ...transparency of methods sections, including clear information about reagents, and why other publishers and ...

...undergo recombination, two smaller plasmids are formed. Viral vectors are prone to recombination In lentiviral... Figure 2: An uncut plasmid is expected to form 3 bands: nicked, linear, and supercoiled. Here we...

...January, we’ll be taking name suggestions using this form or the hashtag #BlugeneVirusFriend on Twitter. As...

...reside there. Biotin is a cofactor that in its active form is covalently attached to the active site of key... interactions found in nature. Once the bond is formed it is unaffected by changes in pH, temperature ...light, the arylazide group becomes reactive and forms a link with nearby nucleic acids (McInnes et al....Complex (ABC) immunohistochemistry (IHC). For more information on ABC and IHC check out Thermo Fisher Scientific's...

...Without proper processing, these small DNA strands can form concatemers in later steps. This new NEB kit includes...that both fragments the DNA and prevents it from forming concatemers later. The "FS" stands for Fragmentation...ask myself when I am bombarded with a lot of information. For example, before going to my next workshop...already? What don’t I understand? How does the information I’ll be learning relate to me as an individual...help me avoid being overwhelmed by all the new information, and instead allow me to pay attention and answer...help you think about the most useful pieces of information from the workshop. Future workshops for Addgene...

...tuberculosis. Investigation of multispecies biofilm formed by Pseudomonas spp. and Klebsiella spp. Dr. Devarshi...examine the antibiotic susceptibility of biofilms formed on various structured surfaces. Plasmids expressing...recombination-mediated genetic engineering (find more information on bacterial engineering using CRISPR on our ...spp. The effects of these deletions on biofilm formation and growth will then be measured to link the deleted...deleted genes to specific functions in biofilm formation. The quest for plant compounds to treat complications...diabetes will be expressed from plasmids (find more information on bacterial expression systems here) and the...understanding neuronal networks, their interactions and information processing during this condition is crucial. ...