...possible without double strand breaks (DSBs) or donor DNA templates. How does this work? First, an engineered... genome editing without double-strand breaks or donor DNA." Nature (2019): 1-1. PubMed PMID: 31634902....

...electroporation and AAV donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to...

...This fragment can then be cloned into a gateway donor plasmid which contains compatible attP sites via...Now that your DNA fragment has been cloned into a donor plasmid, it can be rapidly shuttled into any compatible...can clone your gene of interest one time into a donor plasmid (or acquire one that already has your gene...allowing for rapid cloning of PCR products into donor plasmids without the need for restriction enzyme...

...Instrumental Access, Seeding Labs partners with donors to supply surplus lab equipment to carefully-screened...

... functional elements in the human genome, these donor plasmids will help researchers map transcription...

...sgRNA targeting the genomic site of interest. The donor template is supplied separately. To use this system...both plasmids are transformed in E. coli with the donor DNA for editing to take place. Then plasmids are...system contains 4 plasmids: pDonor, pTnsABC, pQCascade and pCutamp.  pDonor carries a customizable cargo...systems, such as PT7, Prha, Ptet, and Para. The pDonor plasmid pRE57-Ter with multiple terminators can...MUCICAT only needs 2 newly constructed plasmids (pDonor and pQCascade) with 4 rounds of transformation ...

...on both the distance and the orientation of the donor and acceptor proteins, which can be difficult to...

...the recombinase handles the installation of the donor sequence. The latest work evolves and engineers ...site. A recombinase integrates a sequence from a donor plasmid containing a complementary site into the...

..., sweat and tears. (Thanks to our healthy blood donor for keeping going while we worked out the cell capture...

...your target DNA, you first electroporate a linear donor DNA substrate (either dsDNA or ssDNA – see below...where the genetic changes occur in a test tube. The donor DNA substrate only requires ~50 nucleotides of homology...

...a standard CFP set. mTFP1 can be used as a FRET donor to mCitrine and mOrange or for live cell imaging...

... zinc using variants of mOrange2 and mCherry as donor and acceptor domains. He says, these new colors ...

... by targeting start codons, splice acceptor and donor sites, and introducing premature stop codons.  ...

...The second vector is a homology directed repair donor plasmid with homology arms and a selection marker...

...These assays can be designed to detect either a donor sequence integrated through homology-directed repair...

...actinin. When tension is high vs. low in the cell the donor and acceptor of a FRET pair on actinin will produce...

...MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver a genetic ...

...quantify, due to small fluorescence changes of the donor and acceptor after proteolytic cleavage. Secondly...

... kb. This system is transfected into cells as a donor plasmid containing the transposon, along with a ...helper plasmid that expresses the transposase. The donor plasmid contains the gene(s) of interest flanked...

...plain-language stories about Fred Hutch research, pieces for donors such as fundraising materials and progress reports...