...induce transcription at CpG regions of various promoters. Other researchers additionally fused a TAL effector...: dCas9-p300 adds H3K27ac marks to histones in promoter and enhancer regions. These marks are associated...Figure 2: dCas9-Tet1 demethylates cytosines at promoters and enhancers. This targeted demethylation is ...Figure 3: dCas9-DNMT3A methylates cytosines at promoters and enhancers. This targeted methylation is associated...-based acetyltransferase activates genes from promoters and enhancers. Nature Biotechnology 33:510–517.... Grant Challen’s lab also created constitutive (pCMV-dCas9-D3A) and Tet-dependent (TetO-dCas9-D3A) constructs...

...Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically...Cas enzyme and gRNA Species of promoter and expression pattern of promoter for Cas enzyme and gRNA Presence...such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB is more effective...activator (such as dCas9-VP64) gRNA(s) targeting promoter elements of target gene Many different activators...dCas9-repressors, gRNAs should be targeted to the promoter driving expression of your gene of interest. For...

...10878/ . Description Vector Element U6 Human U6 promoter drives RNA Polymerase III transcription for generation...transduced cells. hPGK Human phosphoglycerate kinase promoter drives expression of puromycin. Puro R Puromycin...be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab...Note: pLKO.1 could also be packaged using pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For...

...CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV.... window) . Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato... #103006) . Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato...#127847) . Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP...#185136) . Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG...#185137) . Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG...#175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG...

... hybrid LTR promoter (more information on this below). Additional or specialized promoters may also be...internal promoter is provided, gene expression is driven by the 5'LTR, which is a weak promoter and requires...heterologous promoter on the transfer plasmid. Expression of the transgene from this promoter is no longer... and fused to a heterologous enhancer/promoter such as CMV or RSV Return to Top Viral Production The process...pol II, and thus requires an RNA pol II promoter, such as CMV or RSV. What techniques can be used to clone... use a hybrid 5' LTR with a constitutive promoter such as CMV or RSV. 3' LTR in cis Terminates transcription...generation lentiviral plasmids utilize the viral LTR promoter for gene expression, whereas 3rd-generation transfer...

...pC016 - LwCas13a guide expression backbone with U6 promoter has already earned a blue flame. RNA Editing: ...cytosine base editor pCMV-BE3 has been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already...

...Reference Molecular Biology Reference Promoters Promoters Definition A promoter is a region of DNA where transcription...silencers. Promoter Regions There are three main portions that make up a promoter: core promoter, proximal...Proximal Promoter Further upstream from the core promoter you will find the proximal promoter which contains...bind. Distal Promoter The final portion of the promoter region is called the distal promoter which is upstream.... Common Eukaryotic Promoters Used in Research Promoter Expression Description CMV Constitutive Strong...Specific Drosophila promoter containing Gal4 binding sites Bacterial Promoters Promoters in bacteria contain...tryptophan Promoter from E. coli tryptophan operon Ptac Regulated like the lac promoter Hybrid promoter of lac...

...Chien lab (Kwan et al., 2007) and includes four promoters, six fluorophores with nonoverlapping emission...neurodegenerative diseases, the Cole Lab selected promoters for cell types directly linked to disease, including... components can be controlled by a variety of promoters for versatile and robust engineering. These components... activity; protein purification vector  87439  pCMV-HF-BE3  HF-Cas9 (NGG)  Very low off-target activity...

...Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically...Cas enzyme and gRNA Species of promoter and expression pattern of promoter for Cas enzyme and gRNA Presence...such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB is more effective...activator (such as dCas9-VP64) gRNA(s) targeting promoter elements of target gene Many different activators...dCas9-repressors, gRNAs should be targeted to the promoter driving expression of your gene of interest. For...

...Expresses a guide RNA (gRNA) to target human IRF-1 promoter 61080 gRNA-hIRF-1 #12/pSIR Expresses a guide RNA... RNA (gRNA) to target human IRF-1 promoter 62190 3xFLAG-dCas9/pTEF1p-CYC1t Expresses 3xFLAG-dCas9 in budding...expressing dCas9-2xAM and gRNA for human IRF-1 promoter. 98041 Sa-dCas9-NLS-3xFLAG/pcDNA3.1 Expresses ...Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system. 105283 hIRF-1 gRNA #351...Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system. References An enChIP system...:387. doi: 10.1186/s13104-018-3486-3. PubMed . Promoter-associated proteins of EPAS1 identified by enChIP-MS...regions that physically interact with the Pax5 promoter in a chicken B cell line. Fujita T, Kitaura F,...

...containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter Kay 206512 AAV-LK03 AAV-LK03 AAV packaging vector...containing AAV2 rep and LK03 cap and partial AAV2 p5 promoter Kay 206513 AAV-AM AAV-AM AAV packaging vector ...containing AAV2 rep and AM cap and partial AAV2 p5 promoter Kay 209780 pUCmini-iCAP-AAV1.X1 AAV1-X1 non-standard...198014 pCMV-AAV2cap AAV2 For high-level expression of the AAV2 cap gene, suitable for use with pCMV-Rep78...

...green) and heavy (blue) chains as driven by two CMV promoters (orange). Hybridoma-derived V L and V H domain...κ light chain polyA tail sequences (κ pA), a CMV promoter for heavy chain expression, and an ER signal... the p1316 plasmid that contains an upstream CMV promoter for light chain expression, and an ER signal...