Plasmids 101: Inducible Promoters
Type
Blog Post
...promoter is inactive because the activator protein cannot bind. After an inducer binds to the activator protein...direct activation. In this system, the activator rtTA (reverse tetracycline-controlled transactivator) is... are always active and thus referred to as constitutive promoters. Others are only active under specific...OFF state, the promoter is inactive because a bound repressor protein actively prevents transcription. Once...protein, the activator protein can bind to the promoter, turning it ON and initiating transcription.
...derivatives serve as inducing agents to allow promoter activation.
One of the most commonly used prokaryotic promoters...repressor (lacI protein) for transcription to be activated. In the presence of lactose or lactose analog ...