...Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists Series Learn How... careful not to go too far. Is the assignee interested in this task? Don’t give out tasks – where possible...

...minutes at 12,000 × g at 4°C to separate RNA from the rest of the tissue/cell lysate. Pro-Tip Having your samples...

...and screened by NGS to spot check the samples, the rest will be put into a status we call “Archived.” This...

... 180 regions to another or from the brain to the rest of the body. Therefore, by simply looking at how...

...the meeting and then communicate outcomes to the rest of their team. Here's a template for project meetings...

...and techniques. If you are interested in reading more, you can find the rest of the Plasmids 101 posts ...candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli ...

.... The two remaining plasmids in the kit have the rest of the protein in place including FokI endonuclease...new gene variants associated with conditions of interest, then studying those variants in model systems...library in the appropriate combination and do a restriction enzyme digest to cut out the inserts followed...

...their precise location. The end of the heteroduplex rests against the RuvC nuclease domain, which stabilizes...the target DNA strand, makes few contacts with the rest of Cas9 and is highly flexible; it can rotate about...

...Find lentiCRISPR v2.(If you’ve peeked through the rest of the list at this point, you may have noticed ...Zhang lab is used to deliver Cas9 and your gRNA of interest into mammalian cells. This vector was the top ...Zhang lab codon-optimized SpCas9. Using the BbsI restriction site, a pair of annealed oligos can be cloned...

...of R, is capped, and proceeds through U5 and the rest of the provirus. 3’ LTR Terminates transcription...plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is flanked by long terminal...

.... We know; we asked each group to share with the rest of the workshop.  For Figure 1, Addgene chose this...

... an electrical state. This state, known as the resting potential (Vmem), can be measured as the imbalance...

... translate that valuable information back to the rest of my team in Oxford (UK) to help solve those problems...realized it was a great fit for my skillset and interests. As such, I want to be able to share more about...the North America region. She is particularly interested in DNA sequencing tech, and the intersection ...

...target protein remains bound to the column while the rest of the proteins are eluted. Then, the antibodies...isolate antibodies only recognizing your protein of interest: affinity-purified antibodies are isolated from...

...not happen in a vacuum that is isolated from the rest of society or devoid of social context. This “general...process. My role at pgEd I have always been very interested in the societal aspects of science, even while...science communication or policy requires broad interest in issues at the interface of science and society...narrowly-defined research questions in our field of interest. We often communicate in very technical and jargon-filled...jobs and those of their families. Having a broad interest and awareness of issues outside of your field ...them. Researchers currently in academia who are interested in such careers should take advantage of their...Genetics Education Project (pgEd). He is broadly interested in the legal, ethical and policy issues at the...

...preparation should be doing your best to be well-rested and alert the day of the procedure. Mice move much...

...formats helps these statements stand out from the rest of the text. Specific aims The Specific Aims section...Develop a research plan Most grant applications restrict the space available to describe the proposed work...

...paired with dimmer dyes so they don’t “wash out” the rest of the panel and create spreading error. Conversely...

...locus, provided the target is unique compared to the rest of the genome and located immediately 5’ to a protospacer... What genes are important in your phenotype of interest? Many scientists study diseases for which the ...drug-resistance. Mutant cells are treated with a drug of interest and gRNA distribution is analyzed in the drug-...can help you learn a lot about your phenotype of interest. For more detailed information on CRISPR screens...

...our live examples: experiment or analysis. In the rest of this article, we'll explain the thinking behind...