...promoter is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify... to target cells through microinjection or electroporation Transient expression of CRISPR components Expression...may alter protein function when they occur in important protein domains. For gene editing experiments ... addition to off-target activity , it is also important to consider factors that maximize cleavage of ...25408407 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the...facilitates CRISPR/Cas9-based genome editing. Scientific Reports . 5 , 11315. PMID: 26066060 Zuris, J. A., Thompson...

...disorders. The BRAIN Initiative supports the development of a diverse portfolio of biomolecular tools and emphasizes...and antibodies created with support from the BRAIN Initiative below. Support has been identified by depositors...The BRAIN Initiative supports the development of biomolecular tools for neuroscience research. Browse...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal...128485-AAV9 pAAV-CAG-iSeroSnFR-Nlgn Fluorescent reporter for serotonin (dendrite localization tag) Lin ...

...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a...antibodies only bind a single epitope, so it is important to choose a monoclonal antibody that will recognize...using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals due...with conjugating reactions are possible. It is important to research your reaction and buffer before conjugating... the basic steps needed for each application, important controls, and any special considerations to think...which are bacterial proteins that bind to the Fc portion of an antibody. However, they are not suitable ... assay, selection of the fluorophores used is important. It is best to inversely pair the brightness of...

...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...insertions. When designing the donor template, incorporating mutations that prevent further Cas9 cleavage... HDR efficiency. In most applications, a large portion of the Cas9-induced DSBs will still be repaired...the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit experimentally...pairs to the 3’ flap, and the desired edit is incorporated into the DNA by reverse transcription. The edited...applications where orientation of the insert is important. Type V-K CASTs appear to exhibit almost unidirectional...

... cluster in the MBD and NID demonstrating the importance of binding to methylated DNA and the recruitment...syndrome at the molecular level. They are also an important tool for developing therapeutic strategies to ...MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (Link opens in a new window) PMID: 35148843...opens in a new window) MMRRC Repository - NIH-supported mouse and cell line repository (Link opens in ...Protocols.io - protocol sharing site Funding Opportunities (Link opens in a new window) Rett Syndrome Research... purposeful hand use in girls: Rett’s syndrome: report of 35 cases. Ann Neurol . 14, 471–479. (Link opens...

...collection differ based on some important criteria, and it is important to know which library best...mind: CRISPR screening experiments require electroporation to amplify the pooled library and the use of...best suits your particular experiment. Some important variables include: Type of genetic modification - ...Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries 153101-153106 Knockout Yeast Borodina...Knockout Human Superti-Furga 3rd 6 2,609 Human Transporter Knockout Libraries 213695 221409 221410 Knockout...

...NOT Cre. B) This strategy has been expanded to incorporate VCre in order to enable three-feature cell targeting...recognition sequences enables directional control of the portion of the ORF that is sandwiched between the sites...working with INTRSECT recombinases, viruses, and important controls to consider as part of experimental design..., Poulin JF, Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission... a new window) Mingote S, Amsellem A, Kempf A, Rayport S, Chuhma N. 2020. Dopamine-glutamate neuron projections...

...carrier proteins (SLCs) are the largest family of transporters in the human genome with approximately 450 identified...agreement No 777372. This Joint Undertaking receives support from the European Union's Horizon 2020 research...overexpression library of human SLC proteins. Human Transporter Knockout Libraries 213695 221409 221410 Superti-Furga...knockout library targeting human transmembrane transporters. Feedback Please send your feedback to the Resolute...

...Addgene assists importation by including thorough shipping documents and following import procedure, it ...it is the responsibility of the importer to pay for any additional customs fees or taxes, and to complete... that might be required by your country for the import of biological research materials. Please be aware...

...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49411 pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49409 pCFD2-dU6:2gRNA dU6:2 BbsI Injection or in...transcription Virmilion none, need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, ...

...Endogenous Zebrafish Gene Targets Recent published reports have shown that engineered zinc finger nucleases... making targeted mutations in zebrafish. With support from the NIH and guidance from the academic community...Nuclease Expression Vectors for more details). IMPORTANT NOTE: All zinc finger arrays listed show transcriptional...greater in a well-characterized bacterial two-hybrid reporter system but have NOT yet been tested for activity...

...induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. Woltjen Plasmid Mouse...differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl...self-regulating and nonintegrating viral vectors. Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. Jaenisch...Cells and B Lymphocytes to Neural Stem Cells. Cell Reports. 2014 Dec 11;9(5):1673-80. Parmar Fibroblasts Astrocytes...astrocytes by defined transcription factors. Stem Cell Reports. 2015 Jan 13;4(1):25-36. Broccoli Fibroblasts Sensory...

...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...controlled Cre/loxP system that enables precise temporal and spatial control of gene expression in zebrafish...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...

...(sodium/bile acid cotransporter family), member 2 ASBT, ISBT, NTCP2 TAP1 transporter 1, ATP-binding cassette...grouped into tables based on the Gene Lists from Immport . Click the links below to go to the gene table...perforin and granzyme B, two enzymes that create pores within an infected cell membrane, triggering cell...polypeptide FCRG FCGRT Fc fragment of IgG, receptor, transporter, alpha FCRN, alpha-chain HFE hemochromatosis ...FLJ41500, PSF1, RING4, TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP...ADIPQTL1, ADPN, APM-1, APM1, GBP28, adiponectin ADIPOR1 adiponectin receptor 1 ACDCR1, CGI-45, CGI45, FLJ25385... FLJ25385, FLJ42464, PAQR1, TESBP1A ADIPOR2 adiponectin receptor 2 ACDCR2, FLJ21432, MGC4640, PAQR2 ADM...

...Selectable markers Viral expression and packaging Reporters, shRNA expression, transgenics and genome...stable cell lines in which the plasmid is incorporated into the host DNA. For mammalian cells, you ... AdEasy®-1 plasmid Back to Top Reporters, shRNA expression, transgenics and genome ...promoter strength pBV-Luc - Luciferase reporter plasmid with very low basal activity shRNA/RNAi...

...Browse plasmids supporting the evaluation of a diverse set of novel Alzheimer's disease targets....TREAT-AD centers are focused on diversifying the portfolio of drug targets in Alzheimer’s disease (AD) by...molecules for prioritized targets that are valued opportunities for further translational investment. All resources...

...whole range of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...phage attachment sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled...

...with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...

...research opportunities to their students and other members of the MCC community. They support educators...local hub of educators teaching MDH CUREs, and opportunities to mentor future MCC members. Find a regional...

...identify novel interactions or pathways that are important in cellular recognition processes. Laboratories...Babesia microti blood stage proteins P. falciparum sporozoite cell surface and secreted proteins Mouse sperm...protein interaction network and its resolution by spatiotemporal expression profiling. Martin S, Söllner C, ...